Survivin Antibody (1H5) - BSA Free

Novus Biologicals | Catalog # NBP2-22188

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H5

Format

BSA Free
View all Survivin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Survivin expressed in E. Coli.

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Survivin Antibody (1H5) - BSA Free

Western Blot: Survivin Antibody (1H5)BSA Free [NBP2-22188]

Western Blot: Survivin Antibody (1H5)BSA Free [NBP2-22188]

Western Blot: Survivin Antibody (1H5) [NBP2-22188] - Western blot analysis using Survivin Antibody (1H5) [NBP2-22188] against human BIRC5 (AA: 1-142) recombinant protein. (Expected MW is 16 kDa)
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

Immunocytochemistry/ Immunofluorescence: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

Immunocytochemistry/Immunofluorescence: Survivin Antibody (1H5) [NBP2-22188] - Immunofluorescence analysis of MSCS cells using mouse Survivin Antibody (1H5) [NBP2-22188] (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

Immunohistochemistry-Paraffin: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

Immunohistochemistry-Paraffin: Survivin Antibody (1H5) [NBP2-22188] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using mouse monoclonal Survivin Antibody (1H5) [NBP2-22188] with DAB staining.
Flow Cytometry: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

Flow Cytometry: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

Flow Cytometry: Survivin Antibody (1H5) [NBP2-22188] - Flow cytometric analysis of Jurkat cells using mouse Survivin Antibody (1H5) [NBP2-22188] (green) and negative control (red).
Western Blot: Survivin Antibody (1H5)BSA Free [NBP2-22188]

Western Blot: Survivin Antibody (1H5)BSA Free [NBP2-22188]

Western Blot: Survivin Antibody (1H5) [NBP2-22188] - Western blot analysis using [NBP2-22188] against HEK293 (1) and BIRC5 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate. Note: Predicted molecular weight is 16 kDa.
Immunohistochemistry-Paraffin: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

Immunohistochemistry-Paraffin: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

Immunohistochemistry-Paraffin: Survivin Antibody (1H5) [NBP2-22188] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using mouse monoclonal Survivin Antibody (1H5) [NBP2-22188] with DAB staining.
ELISA: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

ELISA: Survivin Antibody (1H5) - BSA Free [NBP2-22188]

ELISA: Survivin Antibody (1H5) [NBP2-22188] - ELISA using Survivin Antibody (1H5) [NBP2-22188]. Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Applications for Survivin Antibody (1H5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Survivin

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Alternate Names

API4, BIRC5

Gene Symbol

BIRC5

Additional Survivin Products

Product Documents for Survivin Antibody (1H5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Survivin Antibody (1H5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Survivin Antibody (1H5) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: Where is the cellular localization of this antibody?

    A: The localization of this antibody is nuclear and cytoplasmic.

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: Where is the cellular localization of this antibody?

    A: The localization of this antibody is nuclear and cytoplasmic.

Showing  1 - 2 of 2 FAQs Showing All
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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail