Survivin Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56167

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Gerbil

Cited:

Human, Mouse, Rat

Predicted:

Bovine (91%), Canine (92%), Feline (92%), Orangutan (100%), Porcine (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Survivin Primary Antibodies »

Product Specifications

Immunogen

This Survivin Antibody was developed against full-length recombinant human survivin protein.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: guinea pig (87%). Rat reactivity reported in scientific literature (PMID: 18358624). Mouse reactivity reported in scientific literature (PMID: 28011476). Human reactivity reported in scientific literature (PMID: 24916171).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Survivin Antibody - BSA Free

Western Blot: Survivin Antibody [NB100-56167]

Western Blot: Survivin Antibody [NB100-56167]

Western Blot: Survivin Antibody [NB100-56167] - Analysis of Survivin using [NB100-56167]. Survivin was detected in the two tumor cell lines, Jurkat (T cell lymphoma) and RS11846 (non-Hodgkin's lymphoma), but not in lysates from normal tissues. Survivin RP, full-length recombinant human survivin protein was used as a positive control. Bands observed slightly lower than the theoretical molecular weight, 16 kDa.
Western Blot: Survivin Antibody [NB100-56167]

Western Blot: Survivin Antibody [NB100-56167]

Survivin-Antibody-Western-Blot-NB100-56167-img0006.jpg
Immunohistochemistry: Survivin Antibody [NB100-56167]

Immunohistochemistry: Survivin Antibody [NB100-56167]

Immunohistochemistry: Survivin Antibody [NB100-56167] - Immunohistochemical analysis of Survivin in formalin-fixed paraffin-embedded human prostate and breast cancer using Survivin Antibody [NB100-56167]. A and A1, prostate cancer at low and high magnification, respectively. B and B1, breast cancer. B1 is a high magnification of an area of metastasis. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56167]

Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56167]

Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56167] - Analysis of Survivin in a formalin-fixed paraffin-embedded human brain tissue microarray using Survivin Antibody [NB100-56167]. A. Differential expression of Survivin in brain tumors from six different patients. A1-A3, high magnification from A. A1, gemistocytic astrocytoma. A2, medulloblastoma. A3, choroid plexus papilloma. Hematoxylin-eosin counterstain.
Survivin Antibody - BSA Free

Survivin in HeLa Human Cell Line.

Survivin was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-Survivin Whole Serum (Catalog # NB100-56167) at a 1:500 dilution overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
Survivin Antibody - BSA Free

Survivin in HeLa Human Cell Line.

Survivin was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-Survivin Whole Serum (Catalog # NB100-56167) at a 1:500 dilution overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
Survivin Antibody - BSA Free

Survivin in HeLa Human Cell Line.

Survivin was detected in immersion fixed HeLa human cervix adenocarcinoma cell line using Rabbit anti-Survivin Whole Serum (Catalog # NB100-56167) at a 1:500 dilution overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
Survivin Antibody - BSA Free

Survivin in CHO-K1 Chinese Hamster Cell Line.

Survivin was detected in immersion fixed CHO-K1 Chinese hamster ovary epithelium cell line using Rabbit anti-Survivin Whole Serum (Catalog # NB100-56167) at a 1:500 dilution overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
Survivin Antibody - BSA Free

Survivin in CHO-K1 Chinese Hamster Cell Line.

Survivin was detected in immersion fixed CHO-K1 Chinese hamster ovary epithelium cell line using Rabbit anti-Survivin Whole Serum (Catalog # NB100-56167) at a 1:500 dilution overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green), and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
Survivin Antibody - BSA Free

Western Blot: Survivin Antibody - BSA Free [NB100-56167] -

Representative Western blots showing the IAP levels in EV derived from individual European American (EA) (N1-N5) & (N7-N9), *N6 and African American (AA) (N1-N9) patients with prostate cancer (PCa).Specific antibodies against Survivin, XIAP, cIAP-1, cIAP-2, and Lamp1 were used for the Western blotting analysis of total exosomal proteins. The blots from both patient groups were processed under identical conditions; Lamp 1 was used as loading control. (*N6, Hispanic.) (Both blots were done side by side in the same gel running and transferring apparatus, blocking, washing buffers, and antibody incubations were done in the same time, in the same incubating trays under the identical exposure to keep the consistencies.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28981528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Survivin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:10-1:500. Use reported in scientific literature (PMID 24916171)

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Survivin

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Alternate Names

API4, BIRC5

Entrez Gene IDs

332 (Human); 11799 (Mouse); 64041 (Rat)

Gene Symbol

BIRC5

UniProt

O15392

Additional Survivin Products

Product Documents for Survivin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Survivin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Survivin Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Survivin Antibody - BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: How was this antibody purified?

    A: This antibody is supplied in whole antisera, which means it comes in an unpurified form.

  • Q: What dilution would you recommend for use in immunohistochemistry using frozen tissue sections?

    A: It is recommended to use a 1:10-1:500 dilution for both frozen and non-frozen sections. Paraffin-embedded sections would require a 1:1000-1:5000 dilution.

  • Q: What is the concentration of this antibody?

    A: As this antibody is unpurified, we cannot measure the exact concentration.

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: How was this antibody purified?

    A: This antibody is supplied in whole antisera, which means it comes in an unpurified form.

  • Q: What dilution would you recommend for use in immunohistochemistry using frozen tissue sections?

    A: It is recommended to use a 1:10-1:500 dilution for both frozen and non-frozen sections. Paraffin-embedded sections would require a 1:1000-1:5000 dilution.

  • Q: What is the concentration of this antibody?

    A: As this antibody is unpurified, we cannot measure the exact concentration.

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: How was this antibody purified?

    A: This antibody is supplied in whole antisera, which means it comes in an unpurified form.

  • Q: What dilution would you recommend for use in immunohistochemistry using frozen tissue sections?

    A: It is recommended to use a 1:10-1:500 dilution for both frozen and non-frozen sections. Paraffin-embedded sections would require a 1:1000-1:5000 dilution.

  • Q: What is the concentration of this antibody?

    A: As this antibody is unpurified, we cannot measure the exact concentration.

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: How was this antibody purified?

    A: This antibody is supplied in whole antisera, which means it comes in an unpurified form.

  • Q: What dilution would you recommend for use in immunohistochemistry using frozen tissue sections?

    A: It is recommended to use a 1:10-1:500 dilution for both frozen and non-frozen sections. Paraffin-embedded sections would require a 1:1000-1:5000 dilution.

  • Q: What is the concentration of this antibody?

    A: As this antibody is unpurified, we cannot measure the exact concentration.

Showing  1 - 4 of 4 FAQs Showing All
View all FAQs for Antibodies

Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail