Synapsin I Antibody (7H10G6) - BSA Free

Novus Biologicals | Catalog # NBP2-61895

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H10G6

Format

BSA Free
View all Synapsin I Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Synapsin I (AA: 362-511) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

74.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synapsin I Antibody (7H10G6) - BSA Free

Western Blot: Synapsin I Antibody (7H10G6)BSA Free [NBP2-61895]

Western Blot: Synapsin I Antibody (7H10G6)BSA Free [NBP2-61895]

Western Blot: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of HepG2 cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunohistochemistry: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunohistochemistry: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of paraffin-embedded testis tissues using SYN1 mouse mAb with DAB staining.
Flow Cytometry: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Flow Cytometry: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Flow Cytometry: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of Hela cells using SYN1 mouse mAb (green) and negative control (red).
Western Blot: Synapsin I Antibody (7H10G6)BSA Free [NBP2-61895]

Western Blot: Synapsin I Antibody (7H10G6)BSA Free [NBP2-61895]

Western Blot: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein. (Expected MW is 41.7 kDa)
Western Blot: Synapsin I Antibody (7H10G6)BSA Free [NBP2-61895]

Western Blot: Synapsin I Antibody (7H10G6)BSA Free [NBP2-61895]

Western Blot: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis using SYN1 mouse mAb against SK-N-SH (1), NIH/3T3 (2), U251 (3), C6 (4), A549 (5), MCF-7 (6), and COS7 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of GC-7901 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of GC-7901 cells using SYN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunocytochemistry/ Immunofluorescence: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

Immunocytochemistry/Immunofluorescence: Synapsin I Antibody (7H10G6) [NBP2-61895] - Analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
ELISA: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

ELISA: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895]

ELISA: Synapsin I Antibody (7H10G6) [NBP2-61895] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Synapsin I Antibody (7H10G6) - BSA Free

Western Blot: Synapsin I Antibody (7H10G6) - BSA Free [NBP2-61895] -

Microglial cells and SYN/PSD95 protein in the hippocampal region of HO rats. (A) Immunofluorescence staining of Iba1+ cells. Iba1 (green) and DAPI (blue). Scale bars, 100 μm for low magnification images and 50 μm for high magnification images; quantification of Iba1+ cells and the cell body area in the hippocampus, n = 4–6, **P < 0.01 vs. sham-operated group. (B) Immunofluorescence staining of Iba1+ and SYN in the hippocampus: SYN (red), Iba1 (green), and DAPI (blue); scale bars, 50 μm, n = 6, **P < 0.01 vs. sham-operated group. (C) Immunofluorescence staining of Iba1+ and PSD95 in the hippocampus: PSD95 (red), Iba1 (green), and DAPI (blue); scale bars, 50 μm, n = 6, **P < 0.01 vs. sham-operated group. (D–F) The protein levels of SYN and PSD95 in the hippocampus, n = 3, *P < 0.05 vs. sham-operated group. All data are presented as the M +/- SEM of each group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36072565), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Synapsin I Antibody (7H10G6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synapsin I

This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.;

Alternate Names

SYN1

Gene Symbol

SYN1

Additional Synapsin I Products

Product Documents for Synapsin I Antibody (7H10G6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Synapsin I Antibody (7H10G6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Citations for Synapsin I Antibody (7H10G6) - BSA Free

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Protocols

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