Synaptophysin Antibody (7H12) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80972
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7H12
Format
Azide and BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human Synaptophysin expressed in E. coli. [UniProt# P08247]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
33.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Synaptophysin Antibody (7H12) - Azide and BSA Free
![Western Blot: Synaptophysin Antibody (7H12)Azide and BSA Free [NBP2-80972]](https://resources.rndsystems.com/images/products/Synaptophysin-Antibody-7H12-Azide-and-BSA-Free-Western-Blot-NBP2-80972-img0001.jpg "Western Blot: Synaptophysin Antibody (7H12)Azide and BSA Free [NBP2-80972]")
Western Blot: Synaptophysin Antibody (7H12)Azide and BSA Free [NBP2-80972]
Western Blot: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972] - Analysis using Synaptophysin mouse mAb against rat brain tissue lysate. Image from the standard format of this antibody.![Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]](https://resources.rndsystems.com/images/products/Synaptophysin-Antibody-7H12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80972-img0002.jpg "Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]")
Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]
Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972] - Analysis of paraffin-embedded human small intestine, myenteric plexus tissues using anti-Synaptophysin mouse mAb. Image from the standard format of this antibody.![Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]](https://resources.rndsystems.com/images/products/Synaptophysin-Antibody-7H12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80972-img0003.jpg "Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]")
Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]
Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972] - Human pancreas (FFPE) stained with Synaptophysin antibody. pH 9 HIER antigen retrieval. Image from verified customer review. Image from the standard format of this antibody.Applications for Synaptophysin Antibody (7H12) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Synaptophysin
Alternate Names
SYP
Gene Symbol
SYP
Additional Synaptophysin Products
Product Documents for Synaptophysin Antibody (7H12) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Synaptophysin Antibody (7H12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
