Synaptopodin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-39100

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Synaptopodin Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SYNPO. The immunogen is located within the last 50 amino acids of SYNPO. Amino Acid Squence: SPSRAWSPRAKQAPRPS

Specificity

At least three isoforms of SYNPO are known to exist; this antibody will detect all three isoforms. SYNPO antibody is predicted to not cross-react with other SYNPO family member proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synaptopodin Antibody - BSA Free

Western Blot: Synaptopodin AntibodyBSA Free [NBP2-39100]

Western Blot: Synaptopodin AntibodyBSA Free [NBP2-39100]

Western Blot: Synaptopodin Antibody [NBP2-39100] - Analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry: Synaptopodin Antibody - BSA Free [NBP2-39100]

Immunohistochemistry: Synaptopodin Antibody - BSA Free [NBP2-39100]

Immunohistochemistry: Synaptopodin Antibody [NBP2-39100] - Mouse brain tissue with SYNPO antibody at 5 ug/mL.
Synaptopodin Antibody - BSA Free

Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] -

Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] - mTOR signaling pathway regulated PKM2 expression & podocyte differentiation.a IHC detection of p-S6 in renal cortex during postnatal development. Scale bar=20 μm. b Representative western immunoblot analysis of p-S6 in renal cortex. c Representative western immunoblot analysis of p-S6 in cultured podocyte during differentiation. d Western immunoblot analysis of p-S6 & PKM2 expression in Tsc1fl/fl & Tsc1−/−-podocytes. e Rapamycin caused a significant reduction in the protein levels of p-S6 & PKM2 in cultured podocyte. f Western immunoblot analysis of nephrin & synaptopodin with 10 nM rapamycin treatment for 14 days. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32393782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Synaptopodin Antibody - BSA Free

Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] -

Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] - Metabolomics analysis revealed higher lactate production in differentiated podocytes.a Immunofluorescence staining for nephrin (red), phalloidine for F-actin (green) & DAPI for nuclear (blue) in undifferentiated podocytes (UDPs) or differentiated podocytes (DPs) as indicated (n = 5). Scale bar=5 μm. b Representative western blotting results of nephrin, podocin & synaptopodin confirm the differentiation of podocytes (n = 3). c Representative map of 1H-NMR spectra in the extracellular medium incubated with the presence of different podocytes (n = 6). UDM: undifferentiated podocyte medium; DM: differentiated podocyte medium. The map shows the significance of metabolites variations between these two classes. Peaks in the positive direction indicate metabolites that are more abundant in the UDM groups. Consequently, metabolites that are more abundant in DM are presented as peaks in the negative direction. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32393782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Synaptopodin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody - BSA Free [NBP2-39100] -

Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody - BSA Free [NBP2-39100] - Immunofluorescence of Synaptopodin in human brain tissue with Synaptopodin antibody at 20 u/mL.
Synaptopodin Antibody - BSA Free

Immunohistochemistry: Synaptopodin Antibody - BSA Free [NBP2-39100] -

Immunohistochemistry: Synaptopodin Antibody - BSA Free [NBP2-39100] - Immunohistochemistry of Synaptopodin in human brain tissue with Synaptopodin antibody at 2.5 u/mL.
Synaptopodin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody - BSA Free [NBP2-39100] -

Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody - BSA Free [NBP2-39100] - Immunofluorescence of Synaptopodin in mouse brain tissue with Synaptopodin antibody at 20 u/mL.

Red: Synaptopodin Antibody
Blue: DAPI staining

Applications for Synaptopodin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Synaptopodin

SYNPO, also known as synaptopodin, is an actin-associated protein in telencephalic dendrites and renal podocytes. SYNPO is tightly associated with the dendritic spine apparatus, and mice lacking the SYNPO gene lack the apparatus. These SYNPO-null mice also demonstrate an impaired ability to express long-term potentiation as well as deficits in spatial memory tasks, indicating that SYNPO is involved in the regulation of synaptic plasticity. Recent studies suggest that SYNPO is linked to neuronal calcium stores and plays a role in the calcium store-associated ability of neurons to undergo long-term plasticity.

Alternate Names

SYNPO

Gene Symbol

SYNPO

UniProt

NP_001159680 (Human)

Additional Synaptopodin Products

Product Documents for Synaptopodin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Synaptopodin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Synaptopodin Antibody - BSA Free

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Protocols

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