Synaptotagmin 4 Antibody (OTI4H3)

Novus Biologicals | Catalog # NBP2-46045

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4H3
View all Synaptotagmin 4 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protei of human SYT4(NP_065834) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

47.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synaptotagmin 4 Antibody (OTI4H3)

Western Blot: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Western Blot: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Western Blot: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Synaptotagmin 4.
Immunocytochemistry/ Immunofluorescence: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Immunocytochemistry/ Immunofluorescence: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Immunocytochemistry/Immunofluorescence: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045] - Analysis of HeLa cells.
Immunohistochemistry: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Immunohistochemistry: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Immunohistochemistry: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunocytochemistry/ Immunofluorescence: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Immunocytochemistry/ Immunofluorescence: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Immunocytochemistry/Immunofluorescence: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Synaptotagmin 4.
Immunohistochemistry: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Immunohistochemistry: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045]

Immunohistochemistry: Synaptotagmin 4 Antibody (OTI4H3) [NBP2-46045] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for Synaptotagmin 4 Antibody (OTI4H3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Synaptotagmin 4

Synaptotagmin 4 may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis

Alternate Names

HsT1192, KIAA1342sytIV, synaptotagmin 4, synaptotagmin IVsynaptotagmin-4, SytIV

Gene Symbol

SYT4

Additional Synaptotagmin 4 Products

Product Documents for Synaptotagmin 4 Antibody (OTI4H3)

Certificate of Analysis

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Product Specific Notices for Synaptotagmin 4 Antibody (OTI4H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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