Syntenin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33661

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human, Bovine

Predicted:

Bovine (100%), Chimpanzee (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Syntenin 1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Syntenin 1. The exact sequence is proprietary.

Localization

Cell junction, focal adhesion, adherens junction, Cell membrane, Peripheral membrane protein, Endoplasmic reticulum membrane, Peripheral membrane protein, Nucleus, Melanosome, Cytoplasm, cytosol, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Syntenin 1 Antibody - BSA Free

Western Blot: Syntenin 1 Antibody [NBP1-33661]

Western Blot: Syntenin 1 Antibody [NBP1-33661]

Western Blot: Syntenin 1 Antibody [NBP1-33661] - HeLa whole cell extracts and HeLa exosome extract (3.5 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody diluted at 1:250. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Syntenin 1 Antibody [NBP1-33661]

Immunocytochemistry/ Immunofluorescence: Syntenin 1 Antibody [NBP1-33661]

Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody [NBP1-33661] - Analysis of methanol-fixed HeLa, using Syntenin 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661]

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661]

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661]

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661]

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Mouse kidney. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661]

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661]

Immunohistochemistry-Paraffin: Syntenin 1 Antibody [NBP1-33661] - Rat fore brain. Syntenin 1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Syntenin 1 Antibody - BSA Free

Immunohistochemistry-Frozen: Syntenin 1 Antibody [NBP1-33661] -

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse brain.
Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:250.
Blue: Fluoroshield with DAPI.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
Syntenin 1 Antibody - BSA Free

Western Blot: Syntenin 1 Antibody [NBP1-33661] -

Zebrafish tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Syntenin 1 Antibody - BSA Free

Immunohistochemistry-Frozen: Syntenin 1 Antibody [NBP1-33661] -

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse brain.
Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (NBP1-33661) diluted at 1:250.
Blue: Fluoroshield with DAPI.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Applications for Syntenin 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Syntenin 1

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

MDA9, MDA-9, melanoma differentiation associated protein-9, Melanoma differentiation-associated protein 9, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, Scaffold protein Pbp1, ST1, SYCLTACIP18, syndecan binding protein (syntenin), Syndecan-binding protein 1, syntenin-1

Gene Symbol

SDCBP

Additional Syntenin 1 Products

Product Documents for Syntenin 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Syntenin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Syntenin 1 Antibody - BSA Free

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Protocols

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FAQs

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