SYT7 Antibody (S275-14) - BSA Free

Novus Biologicals | Catalog # NBP2-22420

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # S275-14

Format

BSA Free
View all SYT7 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7

Localization

Cytoplasmic Vesicle

Specificity

Detects approx 45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at approx 65kD.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SYT7 Antibody (S275-14) - BSA Free

Western Blot: SYT7 Antibody (S275-14) [NBP2-22420]

Western Blot: SYT7 Antibody (S275-14) [NBP2-22420]

Western Blot: SYT7 Antibody (S275-14) [NBP2-22420] - Western Blot analysis of Rat brain lysates showing detection of SYT7 protein using Mouse Anti-SYT7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Primary Antibody: Mouse Anti-SYT7 Monoclonal Antibody (NBP2-22420) at 1:100, 1:250, 1:500, and 1:1000.
Immunocytochemistry/ Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420]

Immunocytochemistry/ Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420]

Immunocytochemistry/Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody (NBP2-22420) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Synaptotagmin-7 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420]

Immunocytochemistry/ Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420]

Immunocytochemistry/Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X.
Immunocytochemistry/ Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420]

Immunocytochemistry/ Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420]

Immunocytochemistry/Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody (NBP2-22420) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Synaptotagmin-7 Antibody. (D) Composite.

Applications for SYT7 Antibody (S275-14) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of Synaptotagmin-7 Antibody was sufficient for detection of Synaptotagmin-7 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SYT7

Synaptotagmins, such as SYT7, are brain-specific calcium-dependent phospholipid-binding proteins that play a role in synaptic exocytosis and neurotransmitter release. See MIM 600782.[supplied by OMIM]

Alternate Names

IPCA-7synaptotagmin 7, MGC150517, prostate cancer associated protein 7, Prostate cancer-associated protein 7, synaptotagmin VIIPCANAP7, synaptotagmin-7, sytVII, SYT-VII

Gene Symbol

SYT7

UniProt

Q9R0N7 (Mouse)

Additional SYT7 Products

Product Documents for SYT7 Antibody (S275-14) - BSA Free

Certificate of Analysis

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Product Specific Notices for SYT7 Antibody (S275-14) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SYT7 Antibody (S275-14) - BSA Free

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Protocols

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