Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 39D

Format

BSA Free
View all T-bet/TBX21 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was raised against TBX21.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse T-bet/TBX21 Antibody (39D) - BSA Free (NBP1-43299) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-T-bet/TBX21 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for T-bet/TBX21 Antibody (39D) - BSA Free

Western Blot: T-bet/TBX21 Antibody (39D)BSA Free [NBP1-43299]

Western Blot: T-bet/TBX21 Antibody (39D)BSA Free [NBP1-43299]

Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.

Applications for T-bet/TBX21 Antibody (39D) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This 39D antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 ug/ml as a starting dilution for western blotting. Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 23691163).

Reviewed Applications

Read 1 review rated 4 using NBP1-43299 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: T-bet/TBX21

The 39D monoclonal antibody reacts with mouse, rat and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Long Name

T-box transcription factor TBX21

Alternate Names

T-PET, Tbet, TBLYM, TBX21

Gene Symbol

TBX21

Additional T-bet/TBX21 Products

Product Documents for T-bet/TBX21 Antibody (39D) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for T-bet/TBX21 Antibody (39D) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for T-bet/TBX21 Antibody (39D) - BSA Free

Customer Reviews for T-bet/TBX21 Antibody (39D) - BSA Free (1)

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Customer Images

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  • T-bet/TBX21 Antibody (39D)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Rat olfactory bulb, mouse olfactory bulb
    Species: Rat
    Verified Customer | Posted 11/03/2017
    T-bet/ TBX21 positive cells (mitral and tufted cells) in the olfactory bulb of organotypic brain slices
    Tissue has been fixed in formalin, blocked in Normal Goat Serum(NGS). Primary antibody was incubated for 48 hours at 4 degrees in 2.5% NGS + 0.1 % Triton X-100 in PBS (1:250 dilution for T-bet/Tbx21)
    T-bet/TBX21 Antibody (39D) - BSA Free NBP1-43299

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Protocols

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FAQs for T-bet/TBX21 Antibody (39D) - BSA Free

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    • Q: Can your TBX21 antibody (Catalog number NBP1-43299) be used for mouse frozen section by IF? I also wonder whether "ICC/IF"means it can only used for cell sample by IF.
      A: Our T-bet/TBX21 antibody with catalogue number NBP1-43299 has been validated in-house for Western blotting and for immunoprecipitation, while IHC and ICC/IF were validated based on citation of the antibody in the publication detailed in the "reviews and publications" section on our product page. For future reference, ICC/IF does indeed refer to immunocytochemistry / immunofluorescence with cells. IHC refers to the use of tissue samples, with IHC-P indicating paraffin-embedded tissue, and IHC-Fr indicating frozen samples.

    • A: We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.
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