Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all T-bet/TBX21 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TBX2. The immunogen is located within the last 50 amino acids of TBX21. Amino Acid Squence: APSPFDKEAEGQFYNYFPN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit T-bet/TBX21 Antibody - BSA Free (NBP2-41235) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for T-bet/TBX21 Antibody - BSA Free

Western Blot: T-bet/TBX21 AntibodyBSA Free [NBP2-41235]

Western Blot: T-bet/TBX21 AntibodyBSA Free [NBP2-41235]

Western Blot: T-bet/TBX21 Antibody [NBP2-41235] - Western blot analysis of TBX21 in 293 cell lysate with TBX21 antibody at 1 ug/ml.
T-bet/TBX21 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody - BSA Free [NBP2-41235] -

Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody - BSA Free [NBP2-41235] - Immunofluorescence of T-bet/TBX21 in 293 cells with T-bet/TBX21 antibody at 5 ug/mL.
T-bet/TBX21 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody - BSA Free [NBP2-41235] -

Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody - BSA Free [NBP2-41235] - Immunofluorescence of T-bet/TBX21 in 293 cells with T-bet/TBX21 antibody at 5 u/mL.

Applications for T-bet/TBX21 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: T-bet/TBX21

Tbx21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq]

Long Name

T-box transcription factor TBX21

Alternate Names

T-PET, Tbet, TBLYM, TBX21

Gene Symbol

TBX21

Additional T-bet/TBX21 Products

Product Documents for T-bet/TBX21 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for T-bet/TBX21 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for T-bet/TBX21 Antibody - BSA Free

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    • A: We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.
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