TACE/ADAM17 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77044

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TACE/ADAM17 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TACE. The immunogen is located within the last 50 amino acids of TACE. Amino Acid Squence: ASFKLQRQNRVDSKETE

Specificity

80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE/ADAM17. Human TACE/ADAM17 has 2 isoforms, including isoform A (824aa, 93kD), isoform B (694aa, 78.5kD). This antibody detects only isoform A. Mouse TACE/ADAM17 has 2 isoforms, long isoform (827aa, 93.1kD) and short isoform (655aa, 73.9kD). Rat TACE/ADAM17 has only one isoform identified so far (827aa, 93kD).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TACE/ADAM17 Antibody - BSA Free

Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - HeLa whole cell lysate. Image from verified customer review.
Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - Analysis of TACE in HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide with TACE antibody at 0.5 ug/mL.
Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 AntibodyBSA Free [NBP1-77044]

Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - IAV via Protein Expression Profile in Cells Lines. Antibodies: TACE and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.
TACE/ADAM17 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] -

Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] - Figure 5 Immunocytochemistry Validation of TACE/ADAM17 in HeLa CellsImmunohistochemical analysis of HeLa cells using anti-TACE/ADAM17 antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
TACE/ADAM17 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] -

Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] - Figure 4 Immunofluorescence Validation of TACE/ADAM17 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE/ADAM17 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

Applications for TACE/ADAM17 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

0.25-2 ug/mL
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Mesenteric artery isolates and endothelial cell lysates

Reviewed Applications

Read 1 review rated 3 using NBP1-77044 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TACE/ADAM17

Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.

Long Name

TNF-alpha Converting Enzyme

Alternate Names

ADAM17, CD156b

Gene Symbol

ADAM17

UniProt

NP_003174 (Human)

Additional TACE/ADAM17 Products

Product Documents for TACE/ADAM17 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TACE/ADAM17 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TACE/ADAM17 Antibody - BSA Free

Customer Reviews for TACE/ADAM17 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hello cell lysate
    Species: Human
    Verified Customer | Posted 09/09/2014
    Western blot for ADAM17
    TACE/ADAM17 Antibody - BSA Free NBP1-77044

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Protocols

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