TACS-XL In Situ Apoptosis Detection Kit - TACS Blue

R&D Systems | Catalog # 4828-30-BK

R&D Systems
Discontinued Product
4828-30-BK has been discontinued. View all TUNEL Assays products.

Key Product Details

Features

Provides a complete kit for the in situ detection of DNA fragmentation.

Key Benefits

  • Robust method for in situ apoptosis detection
  • Uses BrdU-based methods
  • Precise cellular labeling
  • Signal enhancing methods results in high signal-to-noise ratio

Species

Multi-Species

Product Summary for TACS-XL In Situ Apoptosis Detection Kit - TACS Blue

Why Use the TACS-XL In Situ Apoptosis Detection Kit - TACS Blue?
TACS•XL Blue Label Kit is designed for use with fixed frozen or paraffin embedded tissue sections. All the reagents required for the labeling and detection of fragmented DNA and to assist in the identification of apoptotic morphologies are provided including two permeabilization reagents, labeling and detection reagents, stop buffers, TACS Blue Label and Nuclear Fast Red counterstain as well as TACS-Nuclease for generating positive controls with your own samples.

TACS•XL Blue Label Kit is a complete kit for the in situ detection of DNA fragmentation. This modified TUNEL assay based kit on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that are formed during apoptosis and a specific antibody to BrdU resulting in a high signal-to-noise ratio and less sensitivity to protease-induced false positive labeling than digoxigenin or biotin-based kits.
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Scientific Data Images for TACS-XL In Situ Apoptosis Detection Kit - TACS Blue

Mouse Lung Parenchyma Tissue_TACS XL In Situ Apoptosis Kit

DNA Fragmentation in Mouse Lung Parenchyma Tissue Detected using the TACS XL® Blue Kit.

Mouse lung parenchyma tissue was fixed and embedded in paraffin. Five micron sections were prepared and immobilized on slides. The tissue was labeled as describe in the TACS XL kit protocol (Catalog # 4828-30-BK) and analyzed by light microscopy for DNA fragmentation indicated by the blue stained cells. Data was provided courtesy of F. D'Agostini, University of Genova Via A. Pastore, Genova, Italy.

Kit Contents for TACS-XL In Situ Apoptosis Detection Kit - TACS Blue

  • Proteinase K
  • Streptavidin-HRP
  • TACS-Blue Label™
  • TACS-Nuclease
  • TACS-Nuclease Buffer
  • Nuclear Fast Red
  • TACS 2 TdT Labeling Buffer
  • TACS 2 TdT Stop Buffer
  • TdT Enzyme
  • B-dNTP Mix
  • anti-BrdU antibody
  • Strep-Diluent
  • Cytonin

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for TACS-XL In Situ Apoptosis Detection Kit - TACS Blue

Certificate of Analysis

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Citations for TACS-XL In Situ Apoptosis Detection Kit - TACS Blue

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