TACS-XL In Situ Apoptosis Detection Kit - TACS Blue Summary
• Robust method for in situ apoptosis detection
• Uses BrdU-based methods
• Precise cellular labeling
• Signal enhancing methods results in high signal-to-noise ratio
Why Use the TACS-XL In Situ Apoptosis Detection Kit - TACS Blue?
TACS•XL Blue Label Kit is designed for use with fixed frozen or paraffin embedded tissue sections. All the reagents required for the labeling and detection of fragmented DNA and to assist in the identification of apoptotic morphologies are provided including two permeabilization reagents, labeling and detection reagents, stop buffers, TACS Blue Label and Nuclear Fast Red counterstain as well as TACS-Nuclease for generating positive controls with your own samples.
TACS•XL Blue Label Kit is a complete kit for the in situ detection of DNA fragmentation. This modified TUNEL assay based kit on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that are formed during apoptosis and a specific antibody to BrdU resulting in a high signal-to-noise ratio and less sensitivity to protease-induced false positive labeling than digoxigenin or biotin-based kits.
• Proteinase K
• TACS-Blue Label™
• TACS-Nuclease Buffer
• Nuclear Fast Red
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TdT Enzyme
• B-dNTP Mix
• anti-BrdU antibody
For research use only. Not for diagnositic use.
DNA Fragmentation in Mouse Lung Parenchyma Tissue Detected using the TACS XL® Blue Kit. Mouse lung parenchyma tissue was fixed and embedded in paraffin. Five micron sections were prepared and immobilized on slides. The tissue was labeled as describe in the TACS XL kit protocol (Catalog # 4828-30-BK) and analyzed by light microscopy for DNA fragmentation indicated by the blue stained cells. Data was provided courtesy of F. D'Agostini, University of Genova Via A. Pastore, Genova, Italy.
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