TAF15 Antibody (4D71) - BSA Free

Novus Biologicals | Catalog # NBP1-92685

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D71

Format

BSA Free
View all TAF15 Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human TAF15 expressed in and purified from E. coli. [UniProt# Q92804]

Localization

Nucleus. Cytoplasm. Note: Shuttles from the nucleus to the cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TAF15 Antibody (4D71) - BSA Free

Western Blot: TAF15 Antibody (4D71) [NBP1-92685]

Western Blot: TAF15 Antibody (4D71) [NBP1-92685]

Western Blot: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, NBP1-92685, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction.Double band at 56-68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.
TAF15 Antibody (4D71)

Western Blot Shows Human TAF15 Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 human near-haploid cell line and TAF15 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with TAF15 Antibody (Catalog # NBP1-92685) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for TAF15 (75 kDa) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfers of each blot are shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Immunocytochemistry/ Immunofluorescence: TAF15 Antibody (4D71) [NBP1-92685]

Immunocytochemistry/ Immunofluorescence: TAF15 Antibody (4D71) [NBP1-92685]

Immunocytochemistry/Immunofluorescence: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of HeLa cells stained with mouse mAb to TATA-box binding protein associated factor 15, TAF15, NBP1-92685, dilution 1:500 in red, and costained with rabbit pAb to GAPDH, dilution 1:1,000 in green. Blue is Hoechst staining of nuclear DNA. The NBP1-92685 antibody reveals TAF15 protein expressed exclusively in the nuclei of the cells, while GAPDH antibody produces strong cytoplasmic staining.
TAF15 Antibody (4D71)

Detection of TAF15 by Immunoprecipitation.

HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of TAF15 antibody (Catalog # NBP1-92685) pre-coupled to Dynabeads protein G. Samples were washed and processed for Western Blot with TAF15 antibody. For Western Blot, Rb x TAF15 was used at 1/100. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for TAF15 Antibody (4D71) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry

1:500-1:1000

Immunoprecipitation

Application from YCharOS.

Knockout Validated

Application from YCharOS.

Western Blot

1:1000
Application Notes
This TAF15 (4D71) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 68 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TAF15

TAF15 (TAF2N or TAFII68) is a nuclear protein known to associate with a distinct subpopulation of transcription factor IID (TFIID), a multi-subunit complex that nucleates the pre-initiation complex on promoters of on protein-coding genes. TAF15 harbors a transcriptional activation domain, a RNA recognition motif and many Arg-Gly-Gly repeats that participate in RNA binding. TAF15 along with FUS and EWS constitutes the FET (FUS/EWS/TAF15) protein family of heterogeneous nuclear ribonucleoproteins (hnRNPs) class of RNA binding proteins that are multifunctional proteins implicated in numerous aspects of RNA processing/functions. TAF15 is a nuclear protein that shuttle between cytoplasm-nucleus and as RNA/ssDNA-binding protein, it play specific roles during transcription initiation at distinct promoters and enter the preinitiation complex together with the RNA polymerase II. TAF15 as well as other FET member genes are frequently translocated in sarcomas and rare hematopoietic as well as epithelial cancers. Moreover, dominant mutations in genes coding for four hnRNPs (TDP-43, FUS/TLS, TAF15, and EWS) are found in familial and sporadic cases of ALS and a chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC).

Alternate Names

68kDa, hTAFII68, Npl3, RBP56TAFII68, RNA-binding protein 56, TAF(II)68, TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF2NRBP56/CSMF fusion, TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD(RNA-binding protein 56), TATA box-binding protein-associated factor 2N (RNA-binding protein 56), TATA-binding protein-associated factor 2N, TBP-associated factor 15,68 kDa TATA-binding protein-associated factor

Entrez Gene IDs

8148 (Human)

Gene Symbol

TAF15

UniProt

Q92804 (Human)

Additional TAF15 Products

Product Documents for TAF15 Antibody (4D71) - BSA Free

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Product Specific Notices for TAF15 Antibody (4D71) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TAF15 Antibody (4D71) - BSA Free

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Protocols

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