Tafazzin/TAZ Antibody (1B10) - Azide and BSA Free

Novus Biologicals | Catalog # H00006901-M12

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1B10

Format

Azide and BSA Free
View all Tafazzin/TAZ Primary Antibodies »

Product Specifications

Immunogen

TAZ (AAH11515, 1 a.a. ~ 262 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPLHVKWPFPAVPPLTWTLASSVVMGLVGTYSCFWTSEWAQAEAGPPGYPCPAGGILKLRHIWNLKLMRWTPAAADICFTKELHSHFFSLGKCVPVCRGDGVYQKGMDFILEKLNHGDWVHIFPEGIGRLIAECHLNPIILPLWHVGEPGDGDREMASGVGGLGLPLVPGCPAPPHVWPSVHCAAGMNDVLPNSPPYFPRFGQKITVLIGKPFSALPVLERLRAENKSAVEMRKALTDFIQEEFQHLKTQAEQLHNHLQPGR

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28515468).

Specificity

TAZ (1B10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Tafazzin/TAZ Antibody (1B10) - Azide and BSA Free

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Tafazzin-TAZ-Antibody-1B10-Western-Blot-H00006901-M12-img0010.jpg
Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Analysis of TAZ expression in PC-12 (Cat # L012V1).
Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Analysis of TAZ expression in SW-13 (Cat # L005V1).
Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Analysis of TAZ expression in human uterus myoma.
Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Analysis of TAZ expression in human kidney.
ELISA: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

ELISA: Tafazzin/TAZ Antibody (1B10) [H00006901-M12]

ELISA: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Detection limit for recombinant GST tagged TAZ is approximately 3ng/ml as a capture antibody.
Tafazzin/TAZ Antibody (1B10)

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] -

Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Taz protein expression is higher in the soleus compared with plantaris & is upregulated in response to both overload (OVL) & unload (UNL) stimuli. (a) Taz protein expression (~27 kDa) in the sham plantaris & soleus muscles. (b) Taz protein in the OVL plantaris compared with sham. (c) Taz protein in the UNL soleus compared with sham. *p ≤ 0.05 using a Student’s t-test (a, two-tailed) or paired t-test (b & c, two-tailed), n = 6 per group.  Please refer to Fig. S4 for full length Western blots. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28515468), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Tafazzin/TAZ Antibody (1B10) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for ELISA.

Reviewed Applications

Read 1 review rated 3 using H00006901-M12 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tafazzin/TAZ

This gene encodes a protein that is expressed at high levels in cardiac and skeletal muscle. Mutations in this gene have been associated with a number of clinical disorders including Barth syndrome, dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibroelastosis, and left ventricular noncompaction (LVNC). Multiple transcript variants encoding different isoforms have been described. A long form and a short form of each of these isoforms is produced; the short form lacks a hydrophobic leader sequence and may exist as a cytoplasmic protein rather than being membrane-bound. Other alternative transcripts encoding additional isoforms have been described but the full-length nature of these transcripts is not known.

Alternate Names

BTHSEFE, cardiomyopathy, dilated 3A (X-linked), CMD3A, EFE2FLJ27390, G4.5endocardial fibroelastosis 2, LVNCX, Protein G4.5, tafazzin, Taz1, XAP-2

Entrez Gene IDs

6901 (Human); 66826 (Mouse); 363521 (Rat)

Gene Symbol

TAFAZZIN

OMIM

300069 (Human)

UniProt

AAH11515 (Human)

Additional Tafazzin/TAZ Products

Product Documents for Tafazzin/TAZ Antibody (1B10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Tafazzin/TAZ Antibody (1B10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Tafazzin/TAZ Antibody (1B10) - Azide and BSA Free

Customer Reviews for Tafazzin/TAZ Antibody (1B10) - Azide and BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Soleus
    Species: Mouse
    Verified Customer | Posted 08/26/2016
    Tafazzin expression in mouse soleus
    Tafazzin/TAZ Antibody (1B10) - Azide and BSA Free H00006901-M12

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