TARS Antibody (1A9) - Azide and BSA Free

Novus Biologicals | Catalog # H00006897-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A9

Format

Azide and BSA Free
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Product Specifications

Immunogen

TARS (NP_689508, 44 a.a. ~ 143 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RAELNPWPEYIYTRLEMYNILKAEHDSILAEKAEKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLK

Specificity

TARS - threonyl-tRNA synthetase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TARS Antibody (1A9) - Azide and BSA Free

Immunohistochemistry-Paraffin: TARS Antibody (1A9) [H00006897-M01]

Immunohistochemistry-Paraffin: TARS Antibody (1A9) [H00006897-M01]

TARS-Antibody-1A9-Immunohistochemistry-Paraffin-H00006897-M01-img0006.jpg
Immunohistochemistry-Paraffin: TARS Antibody (1A9) [H00006897-M01]

Immunohistochemistry-Paraffin: TARS Antibody (1A9) [H00006897-M01]

TARS-Antibody-1A9-Immunohistochemistry-Paraffin-H00006897-M01-img0007.jpg
Western Blot: TARS Antibody (1A9) [H00006897-M01]

Western Blot: TARS Antibody (1A9) [H00006897-M01]

Western Blot: TARS Antibody (1A9) [H00006897-M01] - TARS monoclonal antibody (M01), clone 1A9 Analysis of TARS expression in HeLa.
Immunohistochemistry-Paraffin: TARS Antibody (1A9) [H00006897-M01]

Immunohistochemistry-Paraffin: TARS Antibody (1A9) [H00006897-M01]

Immunohistochemistry-Paraffin: TARS Antibody (1A9) [H00006897-M01] - Analysis of monoclonal antibody to TARS on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.
TARS Antibody (1A9)

Immunohistochemistry: TARS Antibody (1A9) [H00006897-M01] -

Immunohistochemistry: TARS Antibody (1A9) [H00006897-M01] - TARS expression co-localized with VEGF & in leukocytes. Tissues were stained by IHC as in Figure 2. Shown are 40x images of serial sections stained with (A,B) No primary Ab as a negative control (C,D) TARS or (E,F) VEGF. Bottom panels show examples of TARS staining in infiltrating leukocytes. Bar = 50 μm. See Additional File 3 for supporting images. Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-620), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TARS Antibody (1A9)

Immunohistochemistry: TARS Antibody (1A9) [H00006897-M01] -

Immunohistochemistry: TARS Antibody (1A9) [H00006897-M01] - TARS expression increases with stage of ovarian cancer. A. Tissues were stained for TARS by immunohistochemistry & counter-stained with Mayers’ hematoxylin. Images were scored blindly by 2 independent investigators & tumor identification was later confirmed by a pathologist (SLM). Shown are 10x images from (1) normal ovary & examples of TARS score increasing with extent of disease (2–4), Bar = 100 μm. B. Graph representing average scores where high stage is ≥ stage 3; statistical significance determined by one-way ANOVA. For more detailed statistics, see Table 1 for Pearson’s Correlations. C. Shown is a graph of individual patient TARS tumor scores grouped according to FIGO stage of disease (i.e. Stage 3.75 = FIGO 3C). Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-620), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TARS Antibody (1A9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TARS

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family

Alternate Names

cytoplasmic, EC 6.1.1.3, MGC9344, Threonine--tRNA ligase, threonyl-tRNA synthetase, threonyl-tRNA synthetase, cytoplasmic

Entrez Gene IDs

6897 (Human)

Gene Symbol

TARS1

OMIM

187790 (Human)

Additional TARS Products

Product Documents for TARS Antibody (1A9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TARS Antibody (1A9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TARS Antibody (1A9) - Azide and BSA Free

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Protocols

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FAQs

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