Tau Antibody

Novus Biologicals | Catalog # NBP2-25163

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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Equine

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY
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Product Specifications

Immunogen

Recombinant full length version of the shortest human Tau isoform purified from E. coli

Localization

Cytoplasm > cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm > cytoskeleton. Cell projection > axon. Note: Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.

Marker

pan-Neuronal Marker

Specificity

This antibody is expected to bind to all Tau isoforms

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

48-67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tau Antibody

Western Blot: Tau Antibody [NBP2-25163]

Western Blot: Tau Antibody [NBP2-25163]

Western Blot: Tau Antibody [NBP2-25163] - Analysis of tissue lysates using chicken pAb to MAP-tau, NBP2-25163, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as multiple isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot in the range from 48 kDa to 67 kDa.
Immunocytochemistry/ Immunofluorescence: Tau Antibody [NBP2-25163]

Immunocytochemistry/ Immunofluorescence: Tau Antibody [NBP2-25163]

Immunocytochemistry/Immunofluorescence: Tau Antibody [NBP2-25163] - Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with chicken pAb to MAP-tau, NBP2-25163, dilution 1:2,000 in green, and costained with mouse mAb to MAP2, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The NBP2-25163 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.

Applications for Tau Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:10000
Application Notes
This Tau antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where multiple bands can be seen in the range 48-67 kDa, with an additional band at 100kDa, corresponding to the 9 different isoforms of Tau.

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as a concentrated total IgY preparation from egg yolk, dialyzed against PBS with added preservative.

Preservative

0.035% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tau

Tau is a relatively low molecular weight member of the microtubule associated protein or MAP family. Most of these proteins were discovered since microtubules can be polymerized in cell homogenates and pelleted out by centrifugation, typically taking MAP proteins with them. This early work showed that the Tau protein facilitated the polymerization of microtubules, and was therefore named after the Greek letter tau, since it promoted tubule formation. Tau is heavily concentrated in axons of neurons, but may also be found in dendrites and in some non-neuronal cells. Much interest has focused on Tau as it is a major component of the neurofibrillary tangles of Alzheimer's disease. Tau in neurofibrillary tangles is typically heavily and aberrantly phosphorylated, and it is believed that phosphorylation may be involved in tangle formation. In addition, numerous different point mutations in the Tau gene are causative of Frontotemporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17). There is one mammalian Tau gene which produces at least 9 different proteins by alternate transcription. In the central nervous system 6 isoforms predominant which either include or do not include three short exon coded inserts. These proteins range in size from 352-441 amino acids and run on SDS-PAGE gels as multiple bands ranging from 48-67 kDa. In peripheral nervous system a form called "big tau" predominates, another alternate transcript which includes a 254 amino acid insert. This form of Tau is found in small amounts in the brain also, in cranial nerve motor nuclei and sensory processes of most sensory ganglia, and runs on SDS-PAGE with an apparent molecular weight of 100 kDa. Each Tau protein contains 3 or 4 copies of an 18 amino acid peptide which are responsible for binding to the microtubules and are similar to those found in MAP2 and other members of the MAP family. Tau is a highly charged acidic protein with few hydrophobic residues which belongs to the family of "intrinsically unstructured proteins". As with GAP43, MARCKS and several other similar proteins, Tau isoforms run on SDS-PAGE much more slowly than expected from their actual molecular weight. This antibody was made against a recombinant construct expressed in and purified from E. coli and which corresponded to the shortest version of the various Tau proteins, so this antibody is expected to bind to all Tau isoforms.

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Gene Symbol

MAPT

OMIM

157140 (Human)

Additional Tau Products

Product Documents for Tau Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Tau Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Tau Antibody

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  • Q: Could you let us know the buffer formulation of #NBP2-25163?

    A: The buffer formulation for this product is 1x PBS with 10mM sodium azide added as a preservative.

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