Tau [p Thr217] Antibody

Novus Biologicals | Catalog # NBP3-13481

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Tau Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr217 of human Tau. The exact sequence is proprietary.

Modification

p Thr217

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Tau [p Thr217] Antibody

ELISA: Tau [p Thr217] Antibody [NBP3-13481]

ELISA: Tau [p Thr217] Antibody [NBP3-13481]

ELISA: Tau [p Thr217] Antibody [NBP3-13481] - Indirect ELISA analysis performed by coating plate with recombinant partial Tau peptide and Tau (phospho Thr217) peptide (1000-0.003 ng/mL). Coated protein was probed with Tau (phospho Thr217) antibody (NBP3-13481) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.
Tau [p Thr217] Antibody

IHC-P-Tau [p Thr217] Antibody-Rat brain. Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

IHC-P-Tau [p Thr217] Antibody-Rat brain. Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Tau [p Thr217] Antibody

Western Blot: Tau [p Thr217] Antibody [NBP3-13481] -

Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody (NBP3-13481) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Tau [p Thr217] Antibody

Immunocytochemistry/ Immunofluorescence: Tau [p Thr217] Antibody [NBP3-13481] -

Tau (phospho Thr217) antibody detects Tau (phospho Thr217) protein at nucleus and cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody (NBP3-13481) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Tau [p Thr217] Antibody

Application
Recommended Usage

ELISA

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tau

Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Tau's association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4).

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Gene Symbol

MAPT

Additional Tau Products

Product Documents for Tau [p Thr217] Antibody

Certificate of Analysis

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Product Specific Notices for Tau [p Thr217] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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