Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Format
Product Specifications
Immunogen
Clonality
Host
Isotype
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TBK1 Antibody - BSA Free
Western Blot: TBK1 AntibodyAzide and BSA Free [NBP2-93921]
Western Blot: TBK1 Antibody [NBP2-93921] - Analysis of extracts of Rat thymus, using TBK1/NAK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.Immunohistochemistry-Paraffin: TBK1 Antibody - Azide and BSA Free [NBP2-93921]
Immunohistochemistry-Paraffin: TBK1 Antibody [NBP2-93921] - Rat testis using TBK1/NAK Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Western Blot: TBK1 AntibodyAzide and BSA Free [NBP2-93921]
Western Blot: TBK1 Antibody [NBP2-93921] - Analysis of extracts of Mouse lung, using TBK1/NAK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.Western Blot: TBK1 AntibodyAzide and BSA Free [NBP2-93921]
Western Blot: TBK1 Antibody [NBP2-93921] - Analysis of extracts of various cell lines, using TBK1/NAK antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.Immunohistochemistry-Paraffin: TBK1 Antibody - Azide and BSA Free [NBP2-93921]
Immunohistochemistry-Paraffin: TBK1 Antibody [NBP2-93921] - Rat kidney using TBK1/NAK Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for TBK1 Antibody - BSA Free
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: TBK1
Long Name
Alternate Names
Gene Symbol
Additional TBK1 Products
Product Documents for TBK1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TBK1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for TBK1 Antibody - BSA Free
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.