TCERG1 Antibody - BSA Free

Novus Biologicals | Catalog # NB300-214

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TCERG1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a portion of human transcription elongation regulator 1 (CA150) encoded within exon 1 (LocusLink ID 10915).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCERG1 Antibody - BSA Free

Western Blot: TCERG1 Antibody [NB300-214]

Western Blot: TCERG1 Antibody [NB300-214]

Western Blot: TCERG1 Antibody [NB300-214] - Detection of Human CA150 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CA150 antibody NB300-214 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: TCERG1 Antibody [NB300-214]

Immunohistochemistry-Paraffin: TCERG1 Antibody [NB300-214]

Immunohistochemistry-Paraffin: TCERG1 Antibody [NB300-214] - Section of human breast carcinoma (left) and mouse squamous cellcarcinoma (right). Antibody: Affinity purified rabbit anti-CA150 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: TCERG1 Antibody [NB300-214]

Western Blot: TCERG1 Antibody [NB300-214]

Western Blot: TCERG1 Antibody [NB300-214] - Nuclear extract (5 ug) from HeLa cells. Antibody used at 0.33 ug/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NBT-BCIP as substrate for 20 min.

Applications for TCERG1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TCERG1

CA150 is a nuclear factor that represses RNA polymerase II (RNAPII) transcription by inhibiting the elongation of transcripts. It has also been shown to be involved in the age onset of Huntington's disease.

Alternate Names

CA150TATA box-binding protein-associated factor 2S, co-activator of 150 kDa, TAF2SMGC133200, TATA box binding protein (TBP)-associated factor, RNA polymerase II, S, 150kD, TATA box-binding protein-associated factor 2S, transcription elongation regulator 1, Transcription factor CA150, Urn1

Entrez Gene IDs

10915 (Human)

Gene Symbol

TCERG1

Additional TCERG1 Products

Product Documents for TCERG1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TCERG1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TCERG1 Antibody - BSA Free

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Protocols

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