TCF-12/HTF4 Antibody (2E9) - Azide and BSA Free
Novus Biologicals | Catalog # H00006938-M01
![Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]](https://resources.rndsystems.com/images/products/TCF-12-HTF4-Antibody-2E9-Western-Blot-H00006938-M01-img0014.jpg "Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2E9
Format
Azide and BSA Free
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Product Specifications
Immunogen
TCF12 (NP_996919, 364 a.a. ~ 453 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCEQSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGH
Specificity
TCF12 - transcription factor 12 (HTF4, helix-loop-helix transcription factors 4)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for TCF-12/HTF4 Antibody (2E9) - Azide and BSA Free
Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]
Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - TCF12 monoclonal antibody (M01), clone 2E9 Analysis of TCF12 expression in Jurkat.![Immunocytochemistry/ Immunofluorescence: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]](https://resources.rndsystems.com/images/products/TCF-12-HTF4-Antibody-2E9-Immunocytochemistry-Immunofluorescence-H00006938-M01-img0006.jpg "Immunocytochemistry/ Immunofluorescence: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]")
Immunocytochemistry/ Immunofluorescence: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]
Immunocytochemistry/Immunofluorescence: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - TCF12 ( Cat# H00006938-M01 ) on HeLa cell. [antibody concentration 10 ug/ml]![Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]](https://resources.rndsystems.com/images/products/TCF-12-HTF4-Antibody-2E9-Immunohistochemistry-Paraffin-H00006938-M01-img0012.jpg "Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]")
Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]
Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of monoclonal antibody to TCF12 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.![Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]](https://resources.rndsystems.com/images/products/TCF-12-HTF4-Antibody-2E9-Western-Blot-H00006938-M01-img0013.jpg "Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]")
Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]
Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of TCF12 over-expressed 293 cell line, cotransfected with TCF12 Validated Chimera RNAi ( Cat # H00006938-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TCF12 monoclonal antibody (M01), clone 2E9 (Cat # H00006938-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]](https://resources.rndsystems.com/images/products/TCF-12-HTF4-Antibody-2E9-Western-Blot-H00006938-M01-img0015.jpg "Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]")
Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01]
Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of TCF12 expression in transfected 293T cell line by TCF12 monoclonal antibody (M01), clone 2E9.Lane 1: TCF12 transfected lysate(75.8 KDa).Lane 2: Non-transfected lysate.Applications for TCF-12/HTF4 Antibody (2E9) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TCF-12/HTF4
Long Name
Transcription Factor 12
Alternate Names
BHLHB20, HEB, HTF4, TCF12
Entrez Gene IDs
6938 (Human)
Gene Symbol
TCF12
OMIM
600480 (Human)
UniProt
Additional TCF-12/HTF4 Products
Product Documents for TCF-12/HTF4 Antibody (2E9) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TCF-12/HTF4 Antibody (2E9) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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