TCF-3/E2A Antibody (5G2) - Azide and BSA Free

Novus Biologicals | Catalog # H00006929-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5G2

Format

Azide and BSA Free
View all TCF-3/E2A Primary Antibodies »

Product Specifications

Immunogen

TCF3 (NP_003191, 545 a.a. ~ 654 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EREKERRVANNARERLRVRDINEAFKELGRMCQLHLNSEKPQTKLLILHQAVSVILNLEQQVRERNLNPKAACLKRREEEKVSGVVGDPQMVLSAPHPGLSEAHNPAGHM

Specificity

TCF3 - transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TCF-3/E2A Antibody (5G2) - Azide and BSA Free

Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01]

Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01]

Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01] - TCF3 monoclonal antibody (M01), clone 5G2 Analysis of TCF3 expression in Hela S3 NE.
Sandwich ELISA: TCF-3/E2A Antibody (5G2) [H00006929-M01] - Detection limit for recombinant GST tagged TCF3 is approximately 0.03ng/ml as a capture antibody.

Applications for TCF-3/E2A Antibody (5G2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TCF-3/E2A

TCF3 / E2A encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Transcription Factor 3/E2-alpha

Alternate Names

bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR

Entrez Gene IDs

6929 (Human)

Gene Symbol

TCF3

OMIM

147141 (Human)

UniProt

NP_003191

Additional TCF-3/E2A Products

Product Documents for TCF-3/E2A Antibody (5G2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TCF-3/E2A Antibody (5G2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TCF-3/E2A Antibody (5G2) - Azide and BSA Free

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Protocols

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FAQs

No product specific FAQs exist for this product.

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