TCTN1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83623

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TCTN1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FSACSVPVVTGDSQFCSQKAVIYSLNFTANPPQRVFELVDQINPSIFCIHITNYKPALSFINPEVPDENNFDTLMKTSDGFTLNAESYVSFTTKLDIPTA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TCTN1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TCTN1 Antibody [NBP1-83623]

Immunocytochemistry/ Immunofluorescence: TCTN1 Antibody [NBP1-83623]

Immunocytochemistry/Immunofluorescence: TCTN1 Antibody [NBP1-83623] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & actin filaments.
Immunohistochemistry-Paraffin: TCTN1 Antibody [NBP1-83623]

Immunohistochemistry-Paraffin: TCTN1 Antibody [NBP1-83623]

Immunohistochemistry-Paraffin: TCTN1 Antibody [NBP1-83623] - Staining of human placenta shows strong cytoplasmic and nuclear positivity in trophoblastic cells.
TCTN1 Antibody - BSA Free Western Blot: TCTN1 Antibody - BSA Free [NBP1-83623]

Western Blot: TCTN1 Antibody - BSA Free [NBP1-83623]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for TCTN1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TCTN1

TCTN1 encodes a member of the tectonic family of secreted and transmembrane proteins. The orthologous gene in mouse is required for formation of most ventral cell types. It functions downstream of smoothened and rab23 to modulate hedgehog signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. Transcript Variant: This variant (3) uses an alternate exon combination in the central coding region and an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 3).

Long Name

Tectonic Family Member 1

Alternate Names

JBTS13, TECT1, Tectonic-1

Gene Symbol

TCTN1

Additional TCTN1 Products

Product Documents for TCTN1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TCTN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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