Teashirt homolog 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87377

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Teashirt homolog 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YGSPFSESSDQLAHFKGSSSREEKEDPQCPDSVSYPQDSLAQIKAVYANLFSESCWSSLALDLKKSGSTTSTNDASQKESSAPTPTPPTCPVSTTGPTTSTPSTSCSSSTSHSSTTSTSSSSGYDWHQAALAKTLQQTSSYGLLPEPS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Teashirt homolog 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Teashirt homolog 1 Antibody [NBP1-87377]

Immunocytochemistry/ Immunofluorescence: Teashirt homolog 1 Antibody [NBP1-87377]

Immunocytochemistry/Immunofluorescence: Teashirt homolog 1 Antibody [NBP1-87377] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Teashirt homolog 1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: Teashirt homolog 1 Antibody - BSA Free [NBP1-87377]

Chromatin Immunoprecipitation-exo-Seq: Teashirt homolog 1 Antibody - BSA Free [NBP1-87377]

ChIP-Exo-Seq composite graph for Anti-TSHZ1 (NBP1-87377) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
Western Blot: Teashirt homolog 1 Antibody [NBP1-87377]

Western Blot: Teashirt homolog 1 Antibody [NBP1-87377]

Western Blot: Teashirt homolog 1 Antibody [NBP1-87377] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-574
Immunohistochemistry-Paraffin: Teashirt homolog 1 Antibody [NBP1-87377]

Immunohistochemistry-Paraffin: Teashirt homolog 1 Antibody [NBP1-87377]

Immunohistochemistry-Paraffin: Teashirt homolog 1 Antibody [NBP1-87377] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.

Applications for Teashirt homolog 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Teashirt homolog 1

Teashirt homolog 1 encodes a colon cancer antigen that was defined by serological analysis of recombinant cDNA expression libraries. The encoded protein is a member of the teashirt C2H2-type zinc-finger protein family and may be involved in transcriptional regulation of developmental processes.

Alternate Names

Antigen NY-CO-33, NY-CO-33, SDCCAG33, Serologically defined colon cancer antigen 33teashirt family zinc finger 1, teashirt homolog 1, teashirt zinc finger homeobox 1, TSH1teashirt zinc finger 1

Gene Symbol

TSHZ1

Additional Teashirt homolog 1 Products

Product Documents for Teashirt homolog 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Teashirt homolog 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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