Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.
Testosterone Antibody (4E1G2)
Novus Biologicals | Catalog # NBP2-45187
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Radioimmunoassay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4E1G2
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Product Specifications
Immunogen
Testosterone 3 CMO conjugated to BSA
Localization
Secreted and cytoplasmic
Specificity
This monoclonal antibody is highly specific to testosterone. Its affinity constant for testosterone is ~1010M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
288.42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11603)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Testosterone Antibody (4E1G2)
![Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) [NBP2-45187]](https://resources.rndsystems.com/images/products/Testosterone-Antibody-4E1G2-Immunohistochemistry-Paraffin-NBP2-45187-img0001.jpg "Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) [NBP2-45187]")
Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) [NBP2-45187]
Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) [NBP2-45187] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Testosterone monoclonal antibody (4E1G2).Applications for Testosterone Antibody (4E1G2)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.25-0.5 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Testosterone
Alternate Names
17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone
Additional Testosterone Products
Product Documents for Testosterone Antibody (4E1G2)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Testosterone Antibody (4E1G2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Testosterone Antibody (4E1G2)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Testosterone Antibody (4E1G2)
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Q: Does R&D Systems® Testosterone Parameter Assay Kit, Catalog # KGE010, detect free or total testosterone?
A: 'Free' testosterone is typically defined as testosterone which is bioavailable, and not bound to sex hormone binding globulin (SHBG). Interference testing performed by spiking SHBG into testosterone-containing samples, demonstrates interference in testosterone detection with Catalog # KGE010, when the concentration of SHBG is greater than 100 ng/ml. (For further details on interference testing, please refer to the "Specificity" section in the product insert.) Assays for 'total' testosterone measure both 'free' testostrone as well as testosterone bound to SHBG. Since KGE010 does not detect testosterone bound to SHBG, this assay does not measure 'total' testosterone.
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