![Western Blot: TET3 Antibody [NBP2-20602]](https://resources.rndsystems.com/images/products/TET3-Antibody-Western-Blot-NBP2-20602-img0013.jpg "Western Blot: TET3 Antibody [NBP2-20602]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Xenopus
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Cited:
Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chemotaxis, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.
Reactivity Notes
Use in Rat reported in scientific literature (PMID:34246869)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
179 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TET3 Antibody
Western Blot: TET3 Antibody [NBP2-20602]
TET3-Antibody-Western-Blot-NBP2-20602-img0013.jpg![Immunoprecipitation: TET3 Antibody [NBP2-20602]](https://resources.rndsystems.com/images/products/TET3-Antibody-Immunoprecipitation-NBP2-20602-img0002.jpg "Immunoprecipitation: TET3 Antibody [NBP2-20602]")
Immunoprecipitation: TET3 Antibody [NBP2-20602]
Immunoprecipitation: TET3 Antibody [NBP2-20602] - Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 ug of TET3 antibody 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] -
Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, & Tet3. a, b Both human (h) & mouse (m) BMMSCs expressed Tet1, Tet2, & Tet3, as assessed by western blotting (a) & qPCR (b). c Immunocytofluorescent staining showed that CD146-positive BMMSCs expressed Tet1, Tet2, & Tet3. Scale bar, 50 μm. Results are from three independent experiments. ***p <0.001; p values calculated using two-tailed Student's t test (mean ± SD) Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-04464-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Western Blot: TET3 Antibody [NBP2-20602] -
Western Blot: TET3 Antibody [NBP2-20602] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (NBP2-20602) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: TET3 Antibody [NBP2-20602] -
Western Blot: TET3 Antibody [NBP2-20602] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (NBP2-20602) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] -
TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET3 protein stained by TET3 antibody [C3], C-term (NBP2-20602) diluted at 1:400.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Applications for TET3 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Assay dependent
Flow Cytometry
Reported in scientific literature (PMID:34246869)
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry Whole-Mount
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:500-1:1000
Western Blot
1:1000-1:10000
Reviewed Applications
Read 1 review rated 4 using NBP2-20602 in the following applications:
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TET3
Alternate Names
EC 1.14.11.n2, hCG_40738, KIAA0401, methylcytosine dioxygenase TET3, MGC22014, probable methylcytosine dioxygenase TET3, tet oncogene family member 3
Gene Symbol
TET3
UniProt
Additional TET3 Products
Product Documents for TET3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TET3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for TET3 Antibody
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Application: Western BlotSample Tested: MOUSE FIBROBLASTSpecies: MouseVerified Customer | Posted 10/03/2014tet1 & tet2 expression in nuclear and cytopasmic extract after drug treatment at different time points
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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