Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TFF3 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3. The immunogen is located within the last 50 amino acids of TFF3. Amino Acid Squence: ECNNRGCCFDSRIPGV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (81%), Pig (81%).

Specificity

TFF3 antibody is predicted to not cross-react with other TFF family members.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TFF3 Antibody - BSA Free (NBP1-76514) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-TFF3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TFF3 Antibody - BSA Free

Immunohistochemistry-Paraffin: TFF3 Antibody - BSA Free [NBP1-76514]

Immunohistochemistry-Paraffin: TFF3 Antibody - BSA Free [NBP1-76514]

Immunohistochemistry-Paraffin: TFF3 Antibody [NBP1-76514] - in human small intestine tissue with TFF3 antibody at 2.5 ug/mL.
TFF3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TFF3 Antibody - BSA Free [NBP1-76514] -

Immunocytochemistry/ Immunofluorescence: TFF3 Antibody - BSA Free [NBP1-76514] - Immunocytochemistry of TFF3 in HeLa cells with TFF3 antibody at 2 ug/ml.
TFF3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TFF3 Antibody - BSA Free [NBP1-76514] -

Immunocytochemistry/ Immunofluorescence: TFF3 Antibody - BSA Free [NBP1-76514] - Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 ug/mL.
TFF3 Antibody - BSA Free

Western Blot: TFF3 Antibody - BSA Free [NBP1-76514] -

Western Blot: TFF3 Antibody - BSA Free [NBP1-76514] - Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
TFF3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TFF3 Antibody - BSA Free [NBP1-76514] -

Immunocytochemistry/ Immunofluorescence: TFF3 Antibody - BSA Free [NBP1-76514] - Immunofluorescence of TFF3 in human small intestine tissue with TFF3 antibody at 20 ug/ml.

Red: TFF3 Antibody
Blue: DAPI staining

Applications for TFF3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2-20 ug/mL

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFF3

Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Gene Symbol

TFF3

UniProt

NP_003217 (Human)

Additional TFF3 Products

Product Documents for TFF3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TFF3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Inflammatory Mediators

Citations for TFF3 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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