TFIIB Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-34143

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TFIIB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LPRDTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFIIB Antibody - BSA Free

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143] - Staining in human gallbladder and pancreas tissues using anti-GTF2B antibody. Corresponding GTF2B RNA-seq data are presented for the same tissues.
Western Blot: TFIIB Antibody [NBP2-34143]

Western Blot: TFIIB Antibody [NBP2-34143]

Western Blot: TFIIB Antibody [NBP2-34143] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-GTF2B antibody. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: TFIIB Antibody [NBP2-34143]

Immunocytochemistry/ Immunofluorescence: TFIIB Antibody [NBP2-34143]

Immunocytochemistry/Immunofluorescence: TFIIB Antibody [NBP2-34143] - Staining of human cell line HEK 293 shows localization to nucleus & nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143] - Staining of human gallbladder shows high expression.
Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143] - Staining of human pancreas shows low expression as expected.
TFIIB Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: TFIIB Antibody - BSA Free [NBP2-34143]

Chromatin Immunoprecipitation-exo-Seq: TFIIB Antibody - BSA Free [NBP2-34143]

ChIP-Exo-Seq composite graph for Anti-GTF2B (NBP2-34143) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for TFIIB Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFIIB

TFIIB encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq]

Alternate Names

general transcription factor IIB, General transcription factor TFIIB, S300-II, TFIIBTF2BRNA polymerase II transcription factor IIB, transcription initiation factor IIB

Gene Symbol

GTF2B

UniProt

Q00403 (Human)

Additional TFIIB Products

Product Documents for TFIIB Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TFIIB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TFIIB Antibody - BSA Free

There are currently no reviews for this product. Be the first to review TFIIB Antibody - BSA Free and earn rewards!

Have you used TFIIB Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies