Key Product Details

Species Reactivity

Human, Rabbit, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # SPM542
View all TGF-alpha Primary Antibodies »

Product Specifications

Immunogen

A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF-alpha (Uniprot: P01135)

Localization

Cytoplasmic and Extracellular (secreted)

Specificity

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this monoclonal antibody is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Theoretical MW

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34418)

Antibody with azide - store at 2 to 8C.

Scientific Data Images for TGF-alpha Antibody (SPM542)

Western Blot: TGF-alpha Antibody (SPM542) [NBP2-34371]

Western Blot: TGF-alpha Antibody (SPM542) [NBP2-34371]

Western Blot: TGF-alpha Antibody (SPM542) [NBP2-34371] - analysis of lysate from HEK293 cells (30ug) using anti-TGF alpha antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: TGF-alpha Antibody (SPM542) [NBP2-34371]

Immunohistochemistry-Paraffin: TGF-alpha Antibody (SPM542) [NBP2-34371]

Immunohistochemistry-Paraffin: TGF-alpha Antibody (SPM542) [NBP2-34371] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF alpha Monoclonal Antibody (SPM542)

Applications for TGF-alpha Antibody (SPM542)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34371 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein L or PEG purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TGF-alpha

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Long Name

Transforming Growth Factor alpha

Alternate Names

TGFA, TGFalpha

Gene Symbol

TGFA

UniProt

P01135

Additional TGF-alpha Products

Product Documents for TGF-alpha Antibody (SPM542)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TGF-alpha Antibody (SPM542)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TGF-alpha Antibody (SPM542) (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Lysate from HEK293 cells
    Species: Human
    Verified Customer | Posted 10/10/2014
    Immunoblot for TGFalpha
    TGF-alpha Antibody (SPM542) NBP2-34371

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