TGF-beta RII Antibody (PSH01-50)

Novus Biologicals | Catalog # NBP3-33010

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH01-50 expressed in HEK293
View all TGF-beta RII Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human TGF-beta RII aa 23-166 (Extracellular). (Uniprot: P37173)

Localization

Cell membrane, Membrane raft; Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TGF-beta RII Antibody (PSH01-50)

TGF-beta RII Antibody (PSH01-50)

Western Blot: TGF-beta RII Antibody (PSH01-50) [NBP3-33010]

Western Blot: TGF-beta RII Antibody (PSH01-50) [NBP3-33010] - Western blot analysis of TGF-beta RII on different lysates with Rabbit anti-TGF-beta RII antibody (NBP3-33010) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: A549 cell lysate
Lane 3: HT-29 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 65 kDa
Observed band size: 65-80 kDa

Exposure time: 3 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33010) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Applications for TGF-beta RII Antibody (PSH01-50)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TGF-beta RII

TGF beta Receptor II is a total of three members of the TGFbeta family, TGFbeta1, TGFbeta2 and TGFbeta3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGFbetas mediate their activity by high affinity binding to the type II receptor (TGFbeta RII) transmembrane protein with a cytoplasmic serine-threonine kinase domain. For signaling growth inhibition and early gene responses the type II receptor requires both its kinase activity and association with a TGFbeta-binding protein, designated the type I receptor. Two independent groups have recently described the cloning and sequence analysis of genes encoding TGFbeta type I receptor proteins designated ALK-5 (TbetaR-1) and TSR-1, respectively.

Long Name

Transforming Growth Factor beta Receptor II

Alternate Names

TGF-bRII, TGFbetaRII, TGFBR2

Gene Symbol

TGFBR2

Additional TGF-beta RII Products

Product Documents for TGF-beta RII Antibody (PSH01-50)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TGF-beta RII Antibody (PSH01-50)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for TGF-beta RII Antibody (PSH01-50)

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  • Q:

    Can Recombinant Human TGF-β RII (Catalog # 241-R2) be used as a capture reagent in an ELISA-like assay?

    A:

    Yes, it can, but Recombinant Human TGF-β RII Fc Chimera (Catalog # 341-BR) binds to the plate better and has a higher affinity for the ligand. Therefore, Catalog # 341-BR is recommended to use as a capture reagent in ELISA applications.

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