Mouse TGF-beta RII ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-53660

Novus Biologicals

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.105 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Kit Contents for Mouse TGF-beta RII ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TGF-beta RII

TGF beta Receptor II is a total of three members of the TGFbeta family, TGFbeta1, TGFbeta2 and TGFbeta3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGFbetas mediate their activity by high affinity binding to the type II receptor (TGFbeta RII) transmembrane protein with a cytoplasmic serine-threonine kinase domain. For signaling growth inhibition and early gene responses the type II receptor requires both its kinase activity and association with a TGFbeta-binding protein, designated the type I receptor. Two independent groups have recently described the cloning and sequence analysis of genes encoding TGFbeta type I receptor proteins designated ALK-5 (TbetaR-1) and TSR-1, respectively.

Long Name

Transforming Growth Factor beta Receptor II

Alternate Names

TGF-bRII, TGFbetaRII, TGFBR2

Gene Symbol

TGFBR2

Additional TGF-beta RII Products

Product Documents for Mouse TGF-beta RII ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse TGF-beta RII ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Mouse TGF-beta RII ELISA Kit (Colorimetric)

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  • Q:

    Can Recombinant Human TGF-β RII (Catalog # 241-R2) be used as a capture reagent in an ELISA-like assay?

    A:

    Yes, it can, but Recombinant Human TGF-β RII Fc Chimera (Catalog # 341-BR) binds to the plate better and has a higher affinity for the ligand. Therefore, Catalog # 341-BR is recommended to use as a capture reagent in ELISA applications.

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