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- SpecificityDetects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
- SourceMonoclonal Mouse IgG1 Clone # 1D11
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenBovine bone-derived TGF-beta 1 and TGF-beta 2
- FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelAllophycocyanin
Applications
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Recommended
ConcentrationSample
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Intracellular Staining by Flow Cytometry10 µL/106 cellsSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
|
Intracellular Staining by Flow Cytometry
|
Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-TGF‑ beta 1, 2, 3 APC‑conjugated Monoclonal Antibody (Catalog # IC1835A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TGF-beta 1, 2, 3
TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
- Ayala A. et al. (1992) FASEB J. 6:A1604.
- Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
- Dasch J.R. et al. (1989) J. Immunol. 142:1536.
- Long Name:Transforming Growth Factor beta 1, 2, and 3
- Alternate Names:CED; DPD1; LAP; TGFB; TGFB1; TGFbeta; transforming growth factor, beta 1
Related Research Areas
- Airway Inflammation and Remodeling
- Cancer Biomarkers
- Cytokines and Receptors in Allergy and Asthma
- Cytokines and Receptors in Angiogenesis
- Early Mesodermal Lineage Markers
- Inflammatory Mediators
- Myeloid-derived Suppressor Cells (MDSC)
- Preeclampsia
- TGF-beta Family
- TGF-beta Family Ligands
- Th17 Cells
- Th22 Cells
- Th9 Cells
Isotype Controls
Description
Application
Cat#
Citations
Images
Mouse IgG1 APC-conjugated Antibody
Ctrl IC002A 5View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Staining Reagents
Description
Application
Cat#
Citations
Images
Flow Cytometry Permeabilization/Wash Buffer I (1X)
Flow FC005 4View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Flow Cytometry Fixation Buffer (1X)
Flow FC004 3View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Flow Cytometry Fixation & Permeabilization Buffer Kit I
Flow FC009View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)
Flow FC007View Sizes & Prices
| Catalog# | Size | Price | Stock | Quantity |
|---|---|---|---|---|
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Calculators
Reconstitution Calculator
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
Certificate of Analysis Lookup
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Associated Pathways
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- IL-21 Signaling and its Primary Biological Effects in Different Immune Cell Types
- IL-4 Signaling and its Primary Biological Effects in Different Immune Cell Types
- IL-9 Signaling and its Primary Biological Effects in Different Immune Cell Types
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