TGF-beta 1, 2, 3 APC-conjugated Antibody

Name: TGF-beta 1, 2, 3 APC-conjugated Antibody
Brand: R&D Systems

(2 citations)

Datasheet / CoA / SDS

Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry.

Intracellular Staining by Flow Cytometry

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Product Details

Citations (2)

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Specificity

Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
Source

Monoclonal Mouse IgG₁ Clone # 1D11
Purification

Protein A or G purified from hybridoma culture supernatant
Immunogen

Bovine bone-derived TGF-beta 1 and TGF-beta 2
Formulation

Supplied in a saline solution containing BSA and Sodium Azide.
Label

Allophycocyanin

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry

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Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-TGF‑ beta 1, 2, 3 APC‑conjugated Monoclonal Antibody (Catalog # IC1835A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta 1, 2, 3

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

References:
1. Ayala A. et al. (1992) FASEB J. 6:A1604.
2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.

Long Name:

Transforming Growth Factor beta 1, 2, and 3

Alternate Names:

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species	Applications	Sample Type
IL-10 inhibits neuraminidase-activated TGF-beta and facilitates Th1 phenotype during early phase of infection. Authors: Dutta A, Huang C, Chen T, Lin C, Chiu C, Lin Y, Chang C, He Y Nat Commun, 2015;6(0):6374. Species: Mouse Sample Type: Whole Cells Application: Flow Mucosal tolerance to a combination of ApoB and HSP60 peptides controls plaque progression and stabilizes vulnerable plaque in Apob(tm2Sgy)Ldlr(tm1Her)/J mice. Authors: Mundkur L, Mukhopadhyay R, Samson S, Varma M, Kale D, Chen D, Shivaprasad S, Sivanandan H, Soman V, Lu X, Kakkar V PLoS ONE, 2013;8(3):e58364. Species: Mouse Sample Type: Whole Cells Application: Flow

FAQs

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Isotype Controls

Description Application Cat# Citations Images

Mouse IgG₁ APC-conjugated Antibody

Ctrl IC002A 11

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Staining Reagents

Description Application Cat# Citations Images

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5

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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3

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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 4

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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2

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