TGF‑ beta 1, 2, 3 APC‑conjugated Antibody

Name: TGF‑ beta 1, 2, 3 APC‑conjugated Antibody
Brand: R&D Systems
SKU: IC1835A

R&D Systems | Catalog # IC1835A

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Citations (3)

Product Documents

Discontinued Product

IC1835A has been discontinued. View all TGF-beta 1, 2, 3 products.

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Mouse, Avian - Chicken

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1D11

View all TGF-beta 1, 2, 3 Primary Antibodies »

Product Specifications

Immunogen

Bovine bone-derived TGF-beta 1 and TGF-beta 2

Specificity

Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for TGF‑ beta 1, 2, 3 APC‑conjugated Antibody

Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 APC-conjugated Mono-clonal Antibody (Catalog # IC1835A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for TGF‑ beta 1, 2, 3 APC‑conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: PC‑3 human prostate cancer cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta 1, 2, 3

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

References

Ayala A. et al. (1992) FASEB J. 6:A1604.
Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
Dasch J.R. et al. (1989) J. Immunol. 142:1536.