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TGF-beta 1, 2, 3 APC-conjugated Antibody

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Intracellular Staining by Flow Cytometry TGF-beta 1, 2, 3 APC-conjugated Antibody Intracellular Staining by Flow Cytometry

Intracellular Staining by Flow Cytometry

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  • Specificity
    Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
  • Source
    Monoclonal Mouse IgG1 Clone # 1D11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Bovine bone-derived TGF-beta 1 and TGF-beta 2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Intracellular Staining by Flow Cytometry TGF beta 1/2/3 Antibody (1D11) [Allophycocyanin]
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Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-TGF‑ beta 1, 2, 3 APC‑conjugated Mono­clonal Antibody (Catalog # IC1835A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TGF-beta 1, 2, 3
TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
  • References:
    1. Ayala A. et al. (1992) FASEB J. 6:A1604.
    2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
    3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.
  • Long Name:
    Transforming Growth Factor beta 1, 2, and 3
  • Alternate Names:
    CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. IL-10 inhibits neuraminidase-activated TGF-beta and facilitates Th1 phenotype during early phase of infection.
    Authors: Dutta A, Huang C, Chen T, Lin C, Chiu C, Lin Y, Chang C, He Y
    Nat Commun, 2015;6(0):6374.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Mucosal tolerance to a combination of ApoB and HSP60 peptides controls plaque progression and stabilizes vulnerable plaque in Apob(tm2Sgy)Ldlr(tm1Her)/J mice.
    Authors: Mundkur L, Mukhopadhyay R, Samson S, Varma M, Kale D, Chen D, Shivaprasad S, Sivanandan H, Soman V, Lu X, Kakkar V
    PLoS ONE, 2013;8(3):e58364.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

 Ctrl IC002A 8  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

 Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

 Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

 Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

 Flow FC007 2
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