TGF-beta 1, 2, 3 APC-conjugated Antibody IC1835A: R&D Systems

You are here

Home » TGF-beta 1, 2, 3 » TGF-beta 1, 2, 3 APC-conjugated Antibody

TGF-beta 1, 2, 3 APC-conjugated Antibody

 (2 citations)   
Intracellular Staining by Flow Cytometry TGF-beta 1, 2, 3 APC-conjugated Antibody Intracellular Staining by Flow Cytometry

Intracellular Staining by Flow Cytometry

View Details
  • Specificity
    Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
  • Source
    Monoclonal Mouse IgG1 Clone # 1D11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Bovine bone-derived TGF-beta 1 and TGF-beta 2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Intracellular Staining by Flow Cytometry TGF beta 1/2/3 Antibody (1D11) [Allophycocyanin]
click image to view larger
Detection of TGF‑ beta 1, 2, 3 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-TGF‑ beta 1, 2, 3 APC‑conjugated Mono­clonal Antibody (Catalog # IC1835A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TGF-beta 1, 2, 3
TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
  • References:
    1. Ayala A. et al. (1992) FASEB J. 6:A1604.
    2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
    3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.
  • Long Name:
    Transforming Growth Factor beta 1, 2, and 3
  • Alternate Names:
    CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Applications
Sample Type
  1. IL-10 inhibits neuraminidase-activated TGF-beta and facilitates Th1 phenotype during early phase of infection.
    Authors: Dutta A, Huang C, Chen T, Lin C, Chiu C, Lin Y, Chang C, He Y
    Nat Commun, 2015;6(0):6374.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Mucosal tolerance to a combination of ApoB and HSP60 peptides controls plaque progression and stabilizes vulnerable plaque in Apob(tm2Sgy)Ldlr(tm1Her)/J mice.
    Authors: Mundkur L, Mukhopadhyay R, Samson S, Varma M, Kale D, Chen D, Shivaprasad S, Sivanandan H, Soman V, Lu X, Kakkar V
    PLoS ONE, 2013;8(3):e58364.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
View all Antibody FAQs
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

 Ctrl IC002A 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

 Flow FC005 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation Buffer (1X)

 Flow FC004 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation & Permeabilization Buffer Kit I

 Flow FC009 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

 Flow FC007 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used TGF-beta 1, 2, 3 APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
Product Datasheet

SDS
COA
R&D Systems Guarantee

Related Products

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Associated Pathways

See More

Customers Who Viewed
This Item Also Viewed

Mouse IgG1 APC-conjugated Antibody
 
Flow Cytometry Permeabilization/Wash Buffer I (1X)
 
Flow Cytometry Fixation Buffer (1X)
 
Flow Cytometry Fixation & Permeabilization Buffer Kit I
 

Feedback

Customer Information