TGN38 Antibody (2F7.1)

Novus Biologicals | Catalog # NB300-575

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chinese Hamster, Monkey, Rabbit

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F7.1
View all TGN38 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID: 11408588). Rabbit reactivity reported in scientific literature (PMID: 10514494). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Use in Human reported in scientific literature (PMID:24576880).

Marker

TGN Marker

Specificity

Detects Trans Golgi Network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TGN38 Antibody (2F7.1)

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - HeLa and COS7 cells were rinsed with PBS and fixed with -20C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody diluted 1:100 in PBS containing 0.2% BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA. Following washing, cells were imaged using a Zeiss Axioplan microscope. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining of TGN-38 in NRK cells.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/mL final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining in NS-1 Cells.

Applications for TGN38 Antibody (2F7.1)

Application
Recommended Usage

Flow Cytometry

1:20 - 1:50

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:500
Application Notes
IP usage was reported in scientific literature (PMID: 10514494). WB: Detects an approx. 38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal WB conditions, detects an 85-95 kDa band depending on cell type. IF: Staining of TGN38 in NRK cells results in staining predominantly in the TGN. IF: it has been shown that methanol works best as a fixative.

Reviewed Applications

Read 1 review rated 5 using NB300-575 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, 1 mg/mL BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TGN38

TGN38, also known as TGN46, is localized primarily in the Trans-Golgi network. TGN38 has been reported as being the best available marker for human trans-Golgi network

Long Name

Trans-golgi Network Protein

Alternate Names

TGN46, TGN51, Tgoln1, Tgoln2, Ttgn1

Entrez Gene IDs

10618 (Human); 192152 (Rat)

Gene Symbol

TGOLN2

Additional TGN38 Products

Product Documents for TGN38 Antibody (2F7.1)

Certificate of Analysis

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Download SDS

Product Specific Notices for TGN38 Antibody (2F7.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TGN38 Antibody (2F7.1)

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  • TGN38 Antibody (2F7.1)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: INS-1
    Species: Rat
    Verified Customer | Posted 01/06/2020
    TGN38 Antibody (2F7.1) NB300-575

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