TGN38 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-03495

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all TGN38 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from mouse TGN38 conjugated to blue carrier protein was used as the antigen.

Reactivity Notes

Marmoset (100%).

Marker

TGN Marker

Specificity

TGN38 (or TGN46, TGN48, TGN51 in human).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

PLEASE NOTE: 0.1mg sample size is provided in reconstituted format and the 0.5mg size is provided lyophilized. (Please see reconstitution instuctions).

Scientific Data Images for TGN38 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Mouse embryonic fibroblasts, fixed with 4% PFA washed with PBS, permeabilized with 0.2% Triton X-100. Primary antibody at 1:100 in 3% BSA in PBS. Secondary antibodies incubation for 2 hours. Image from a verified customer review.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody [NBP1-03495]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
TGN38 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody - Azide and BSA Free [NBP1-03495] -

Localization of vesicles with accumulated ceramides in ASC specks by live-cell fluorescence confocal microscopy and cryo-ET.a Distribution of BODIPY TR ceramide (BCer) during ASC speck formation in iBMDMs expressing ASC-mCerulean, or WT iBMDMs stained with FAM-FLICA. Areas of speck formation are boxed. See Supplementary Movie 3 for full time courses. Right, percentage of whole-cell fluorescence that mapped to the area of speck formation, before and after speck formation, t0 and tS (1–3 min and 20–50 min after nigericin addition), respectively. 12 specks from ASC-mCerulean iBMDMs and 11 specks from WT iBMDMs were analyzed. See Source Data File for source data. Scale bars, 10 um. b Immunofluorescence microscopy of LPS-primed WT iBMDMs with or without nigericin stimulation. Anti-NLRP3 partially colocalized with anti-TGN38. The TGN was dispersed following stimulation. Scale bar, 10 um. See Supplementary Movie 4 for a Z-stack series. The images are representative of three independent experiments, with at least 20 cells imaged per experiment. c Immunofluorescence microscopy of LPS-primed ASC-mCerulean iBMDMs with or without nigericin stimulation, and with nigericin stimulation and caspase inhibitor Z-VAD-FMK. There is little overlap between ASC-mCerulean and anti-TGN38 fluorescence. Scale bars, 10 um. The images are representative of three independent experiments, with at least 20 cells imaged per experiment. d 1.36-nm thick virtual tomographic slice within an ASC speck. Scale bar, 200 nm. Lower panel, 3-D segmentation model. e Vesicles from cryo-ET reconstructions of ASC-mCerulean iBMDMs. Scale bar, 100 nm. The histogram shows the vesicle size distribution. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37945612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TGN38 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody - Azide and BSA Free [NBP1-03495] -

Localization of vesicles with accumulated ceramides in ASC specks by live-cell fluorescence confocal microscopy and cryo-ET.a Distribution of BODIPY TR ceramide (BCer) during ASC speck formation in iBMDMs expressing ASC-mCerulean, or WT iBMDMs stained with FAM-FLICA. Areas of speck formation are boxed. See Supplementary Movie 3 for full time courses. Right, percentage of whole-cell fluorescence that mapped to the area of speck formation, before and after speck formation, t0 and tS (1–3 min and 20–50 min after nigericin addition), respectively. 12 specks from ASC-mCerulean iBMDMs and 11 specks from WT iBMDMs were analyzed. See Source Data File for source data. Scale bars, 10 um. b Immunofluorescence microscopy of LPS-primed WT iBMDMs with or without nigericin stimulation. Anti-NLRP3 partially colocalized with anti-TGN38. The TGN was dispersed following stimulation. Scale bar, 10 um. See Supplementary Movie 4 for a Z-stack series. The images are representative of three independent experiments, with at least 20 cells imaged per experiment. c Immunofluorescence microscopy of LPS-primed ASC-mCerulean iBMDMs with or without nigericin stimulation, and with nigericin stimulation and caspase inhibitor Z-VAD-FMK. There is little overlap between ASC-mCerulean and anti-TGN38 fluorescence. Scale bars, 10 um. The images are representative of three independent experiments, with at least 20 cells imaged per experiment. d 1.36-nm thick virtual tomographic slice within an ASC speck. Scale bar, 200 nm. Lower panel, 3-D segmentation model. e Vesicles from cryo-ET reconstructions of ASC-mCerulean iBMDMs. Scale bar, 100 nm. The histogram shows the vesicle size distribution. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37945612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TGN38 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Immunohistochemistry

10-50 ug/ml

Western Blot

10-50 ug/ml
Application Notes
The 0.1 mg size ships reconstituted and the 0.5 mg size ships lyophilized. ICC/IF reactivity reported in (PMID: 27505890) and a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-03495 in the following applications:

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Contains 0.02% benzalkonium chloride.

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGN38

The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways. TGN46 cycles between the trans-Golgi network and the cell surface returning via endosomes. It is thought to be involved in regulating membrane traffic to and from trans-Golgi network. Three splice isoforms are found: TGN46, TGN48 and TGN51. Isoform TGN46 is widely expressed, isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells whilst isoform TGN51 is more abundant in fetal lung and kidney. TGN46 is a human specific protein. The rodent homologue of TGN46 is known as TGN38.

Long Name

Trans-golgi Network Protein

Alternate Names

TGN46, TGN51, Tgoln1, Tgoln2, Ttgn1

Entrez Gene IDs

10618 (Human); 22135 (Mouse); 192152 (Rat)

Gene Symbol

TGOLN2

UniProt

Q62314 (Mouse)

Additional TGN38 Products

Product Documents for TGN38 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TGN38 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TGN38 Antibody - Azide and BSA Free

Customer Reviews for TGN38 Antibody - Azide and BSA Free (1)

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  • TGN38 Antibody
    Name: Anonymous
    Application: immune-fluorescence
    Sample Tested: MEF
    Species: Mouse
    Verified Customer | Posted 08/05/2019
    MEF cells were fixed by 4% PFA in PBS, for 15 min at room temperature. The cells were washed with PBS, and permeabilized with 0.2% Triton X100 in PBS, for 5 min at room temperature. The cells were washed with PBS, and then incubated with the indicated antibodies diluted as 1:100 in 3% BSA in PBS, at 4 degree overnight. On the following day, the cells were washed with PBS, and incubated with corresponding secondary antibodies, for 2 h at room temperature. Then the cells were washed with PBS, and mounted with moviol.
    TGN38 Antibody - Azide and BSA Free NBP1-03495

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