Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all THEM2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human THEM2 (Accession#: NP_060943.1; Met1-Asn140)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for THEM2 Antibody - Azide and BSA Free

Western Blot: THEM2 Antibody [NBP2-97056]

Western Blot: THEM2 Antibody [NBP2-97056]

Western Blot: THEM2 Antibody [NBP2-97056] - Anti-THEM2 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa
Immunohistochemistry-Paraffin: THEM2 Antibody [NBP2-97056]

Immunohistochemistry-Paraffin: THEM2 Antibody [NBP2-97056]

Immunohistochemistry-Paraffin: THEM2 Antibody [NBP2-97056] - Immunochemical staining of human THEM2 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: THEM2 Antibody [NBP2-97056]

Immunohistochemistry-Paraffin: THEM2 Antibody [NBP2-97056]

Immunohistochemistry-Paraffin: THEM2 Antibody [NBP2-97056] - Immunochemical staining of human THEM2 in human hepatoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: THEM2 Antibody [NBP2-97056]

Immunoprecipitation: THEM2 Antibody [NBP2-97056]

Immunoprecipitation: THEM2 Antibody [NBP2-97056] - THEM2 was immunoprecipitated using: Lane A: 0.5 mg Mouse liver tissue Lysate 4 ul anti-THEM2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-THEM2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa

Applications for THEM2 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

0.5-2uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: THEM2

THEM2 encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

acyl-CoA thioesterase 13PNAS-27, EC 3.1.2.-, HT012, hypothalamus protein HT012, MGC4961, THEM2, Thioesterase superfamily member 2acyl-coenzyme A thioesterase 13

Gene Symbol

ACOT13

Additional THEM2 Products

Product Documents for THEM2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for THEM2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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