Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80977
Key Product Details
Species Reactivity
Human, Mouse, Rat, Primate, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 4D6
Format
Azide and BSA Free
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Product Specifications
Immunogen
A synthetic peptide corresponding to residues 56-72 of human Thimet Oligopeptidase. [UniProt# P52888]
Localization
Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free
![Western Blot: Thimet Oligopeptidase/THOP1 Antibody (4D6)Azide and BSA Free [NBP2-80977]](https://resources.rndsystems.com/images/products/Thimet-Oligopeptidase-THOP1-Antibody-4D6-Azide-and-BSA-Free-Western-Blot-NBP2-80977-img0001.jpg "Western Blot: Thimet Oligopeptidase/THOP1 Antibody (4D6)Azide and BSA Free [NBP2-80977]")
Western Blot: Thimet Oligopeptidase/THOP1 Antibody (4D6)Azide and BSA Free [NBP2-80977]
Western Blot: Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free [NBP2-80977] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure. Image from the standard format of this antibody.![Immunohistochemistry: Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free [NBP2-80977]](https://resources.rndsystems.com/images/products/Thimet-Oligopeptidase-THOP1-Antibody-4D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80977-img0002.jpg "Immunohistochemistry: Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free [NBP2-80977]")
Immunohistochemistry: Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free [NBP2-80977]
Immunohistochemistry: Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free [NBP2-80977] - Analysis of Thimet Oligopeptidase in mouse brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.Applications for Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:400
Immunohistochemistry-Paraffin
1:400
Western Blot
1:750-1:3000
Application Notes
This Thimet Oligopeptidase (4D6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~80 kDa. For IHC-P, this antibody has been tested in human, monkey, and rat brain and testis. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.54 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Thimet Oligopeptidase/THOP1
Alternate Names
EP24.15
Gene Symbol
THOP1
Additional Thimet Oligopeptidase/THOP1 Products
Product Documents for Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Thimet Oligopeptidase/THOP1 Antibody (4D6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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