Thrombomodulin/BDCA-3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88133

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Thrombomodulin/BDCA-3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DVISLLLNGDGGVGRRRLWIGLQLPPGCGDPKRLGPLRGFQWVTGDNNTSYSRWARLDLNGAPLCGPLCVAVSAAEATVPSEPIWEEQQCEVKADGFLCEFHFPATCRPLAVEPGAAAAAVSITYG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Thrombomodulin/BDCA-3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunocytochemistry/ Immunofluorescence: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunocytochemistry/Immunofluorescence: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human lung shows strong membranous positivity in pneumocytes and endothelial cells.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human pancreas shows strong membranous positivity in endothelial cells.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human placenta shows moderate membranous positivity in trophoblastic and endothelial cells.
Thrombomodulin/BDCA-3 Antibody - BSA Free Western Blot: Thrombomodulin/BDCA-3 Antibody - BSA Free [NBP1-88133]

Western Blot: Thrombomodulin/BDCA-3 Antibody - BSA Free [NBP1-88133]

Analysis in human lung tissue.

Applications for Thrombomodulin/BDCA-3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thrombomodulin/BDCA-3

CD141/Thrombomodulin is an exclusively endothelial cell surface glycoprotein that forms a 1:1 complex with thrombin. Binding of thrombin to this high affinity receptor alters its specificity toward several substrates. The complex activates protein C approximately 1000 times faster than thrombin alone. Activated protein C degrades clotting factors V and VIII; thus, thrombomodulin converts thrombin into a physiologic anticoagulant. Thrombomodulin is also found in the circulatory and urinary systems, the physiologic significance of this is obscure.

Alternate Names

BDCA-3, BDCA3, CD141, Fetomodulin, THBD, THRM

Gene Symbol

THBD

Additional Thrombomodulin/BDCA-3 Products

Product Documents for Thrombomodulin/BDCA-3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Thrombomodulin/BDCA-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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