Thrombopoietin/THPO Antibody (1B11) - BSA Free

Novus Biologicals | Catalog # NBP2-37498

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1B11

Format

BSA Free
View all Thrombopoietin/Tpo Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Thrombopoietin/THPO expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

37.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Thrombopoietin/THPO Antibody (1B11) - BSA Free

Western Blot: Thrombopoietin/THPO Antibody (1B11)BSA Free [NBP2-37498]

Western Blot: Thrombopoietin/THPO Antibody (1B11)BSA Free [NBP2-37498]

Western Blot: Thrombopoietin/Tpo Antibody (1B11) [NBP2-37498] - Western blot analysis using THPO mAb against human THPO (AA: 250-303) recombinant protein. (Expected MW is 30 kDa)
Flow Cytometry: Thrombopoietin/THPO Antibody (1B11) - BSA Free [NBP2-37498]

Flow Cytometry: Thrombopoietin/THPO Antibody (1B11) - BSA Free [NBP2-37498]

Flow Cytometry: Thrombopoietin/Tpo Antibody (1B11) [NBP2-37498] - Flow cytometric analysis of MCF-7 cells using THPO mouse mAb (green) and negative control (red).
ELISA: Thrombopoietin/THPO Antibody (1B11) - BSA Free [NBP2-37498]

ELISA: Thrombopoietin/THPO Antibody (1B11) - BSA Free [NBP2-37498]

ELISA: Thrombopoietin/Tpo Antibody (1B11) [NBP2-37498] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Thrombopoietin/THPO Antibody (1B11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thrombopoietin/Tpo

Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Alternate Names

MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo

Gene Symbol

THPO

Additional Thrombopoietin/Tpo Products

Product Documents for Thrombopoietin/THPO Antibody (1B11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Thrombopoietin/THPO Antibody (1B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Thrombopoietin/THPO Antibody (1B11) - BSA Free

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  • Q: Why do you sell Thrombopoietin protein from two different expression systems?  Under what circumstances would it be preferred to use the NS0-expressed protein?

    A: The NS0-expressed protein is necessary for the serum-free megakaryocyte colony stimulating assay (Nadine Weich, Genetics Insititute).  In all other assays, it works the same as the Sf-21 derived protein.

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