Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 378120
View all Thrombopoietin/Tpo Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Thrombopoietin/Tpo
Ser22-Thr356 (predicted)
Accession # P40226

Specificity

Detects mouse Thrombopoietin/Tpo in ELISAs and Western blots. In sandwich immunoassays, 0.65% cross-reactivity with NS0-derived recombinant human (rh) Tpo is observed. No significant cross-reactivity or interference with rhEPO, recombinant mouse (rm) G-CSF, rmGM-CSF, rmM-CSF, rmSCF, rmTpo R, or rmVEGF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Thrombopoietin/THPO Antibody (378120) [Biotin]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Thrombopoietin/Tpo

Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Alternate Names

MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo

Gene Symbol

THPO

Additional Thrombopoietin/Tpo Products

Product Documents for Thrombopoietin/THPO Antibody (378120) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Thrombopoietin/THPO Antibody (378120) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Thrombopoietin/THPO Antibody (378120) [Biotin]

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  • Q: Why do you sell Thrombopoietin protein from two different expression systems?  Under what circumstances would it be preferred to use the NS0-expressed protein?

    A: The NS0-expressed protein is necessary for the serum-free megakaryocyte colony stimulating assay (Nadine Weich, Genetics Insititute).  In all other assays, it works the same as the Sf-21 derived protein.

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