Thymidylate Synthase Antibody (SPM453)

Novus Biologicals | Catalog # NBP2-34390

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM453
View all Thymidylate Synthase Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Thymidylate Synthase protein. (Uniprot: P04818 )

Localization

Cytoplasm, Nucleus, Mitochondria

Marker

5-FU Resistance Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34441)

Antibody with azide - store at 2 to 8C.

Scientific Data Images for Thymidylate Synthase Antibody (SPM453)

Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390]

Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390]

Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Thymidylate Synthase Mouse Monoclonal Antibody (SPM453).

Applications for Thymidylate Synthase Antibody (SPM453)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.25-0.5ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 3 using NBP2-34390 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Thymidylate Synthase

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

Long Name

Thymidylate synthase

Alternate Names

EC 2.1.1.45, OK/SW-cl.29, TS, TSase, TYMS

Gene Symbol

TYMS

UniProt

P04818 (Human)

Additional Thymidylate Synthase Products

Product Documents for Thymidylate Synthase Antibody (SPM453)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Thymidylate Synthase Antibody (SPM453)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Thymidylate Synthase Antibody (SPM453) (1)

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Customer Images

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  • Thymidylate Synthase Antibody (SPM453)
    Name: Masashi Okada
    Application: Western Blot
    Sample Tested: Tumor cell lyastes
    Species: Human
    Verified Customer | Posted 09/14/2017
    three tumor cell lysates (20 ug) are loaded. 1/1000 dil Ab in 2% BSA/TBST, incubated for o/n at 4C
    Thymidylate Synthase Antibody (SPM453) NBP2-34390

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