TIF1 alpha Antibody

Novus Biologicals | Catalog # NB100-2596

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation Sequencing

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TIF1 alpha Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human Transcriptional Intermediary Factor 1 Alpha (Tripartite Motif-Containing 24 ) using the numbering given in entry NP_056989.2 (GeneID 8805).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIF1 alpha Antibody

Western Blot: TIF1 alpha Antibody [NB100-2596]

Western Blot: TIF1 alpha Antibody [NB100-2596]

Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 Alpha/TRIM24 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody NB100-2596 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596]

Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596]

Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-TIF1alpha/TRIM24 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation Sequencing: TIF1 alpha Antibody [NB100-2596] - Localization of TIF1 Alpha/TRIM24 Binding Sites by ChIP-sequencing.Chromatin from Breast cancer cell line T47D was immunoprecipitated with anti-TIF1 Alpha/TRIM24 antibody NB100-2596 and analyzed by DNA sequencing. The figure illustrates the peak distribution of TIF1 Alpha/TRIM24 binding within a 250 Kb region of chromosome 19 as detected using anti-TIF1 Alpha/TRIM24 antibody NB100-2596. ChIP-seq validation performed by Active Motif, Carlsbad, CA.
Western Blot: TIF1 alpha Antibody [NB100-2596]

Western Blot: TIF1 alpha Antibody [NB100-2596]

Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 alpha/TRIM24 on HeLa whole cell lysate using NB100-2596. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2597.
Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596]

Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596]

Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for TIF1 alpha Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ChIP-Seq ChIP-Seq 4 ug/30 ug chromatin.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TIF1 alpha

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Long Name

Transcription intermediary factor 1-alpha

Alternate Names

RNF82, TIF1, TIF1-alpha, TIF1A, TRIM24

Entrez Gene IDs

8805 (Human); 21848 (Mouse)

Gene Symbol

TRIM24

UniProt

O15164 (Human)

Additional TIF1 alpha Products

Product Documents for TIF1 alpha Antibody

Certificate of Analysis

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Product Specific Notices for TIF1 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TIF1 alpha Antibody

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Protocols

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