Tight Junction Protein 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85046

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Tight Junction Protein 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGR

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31174067). Analysis of Tight Junction Protein 1 in RIPA whole cell lysates of Mouse primary choroid plexus epithelial cells using anti-Tight Junction Protein 1 antibody verified customer review.

Marker

Intercellular Junctions/Tight Junction Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Tight Junction Protein 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046]

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046]

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining in human cerebral cortex and skeletal muscle tissues using anti-TJP1 antibody. Corresponding TJP1 RNA-seq data are presented for the same tissues.
Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046]

Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046]

Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Analysis of Tight Junction Protein 1 in RIPA whole cell lysates of mouse primary choroid plexus epithelial cells using anti-Tight Junction Protein 1 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85046]

Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85046]

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046]

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046]

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046]

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046]

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human skeletal muscle shows low expression as expected.
Tight Junction Protein 1 Antibody

Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] -

Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Expression of tight junction proteins in cerebellum of rats with mild liver damage. Expression of (A,B) occludin & (C,D) ZO-1 at 2 & 4 weeks was analyzed by Western blot. Values are mean ± SEM of 10–12 rats per group. One-way ANOVA with Tukey’s test was performed for occludin at 2 weeks (F(3,27) = 4.99, p < 0.01) & 4 weeks (F(3,47) = 0.611, p > 0.05) & for ZO-1 at 2 weeks (W(3,23) = 0.715, p > 0.05) & 4 weeks (F(3,43) = 3.698, p < 0.05). Values significantly different from control rats are indicated by asterisks & from CCl4 rats by a. * p < 0.05; a p < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Tight Junction Protein 1 Antibody

Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] -

Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Expression of tight junction proteins in cerebellum of rats with mild liver damage. Expression of (A,B) occludin & (C,D) ZO-1 at 2 & 4 weeks was analyzed by Western blot. Values are mean ± SEM of 10–12 rats per group. One-way ANOVA with Tukey’s test was performed for occludin at 2 weeks (F(3,27) = 4.99, p < 0.01) & 4 weeks (F(3,47) = 0.611, p > 0.05) & for ZO-1 at 2 weeks (W(3,23) = 0.715, p > 0.05) & 4 weeks (F(3,43) = 3.698, p < 0.05). Values significantly different from control rats are indicated by asterisks & from CCl4 rats by a. * p < 0.05; a p < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Tight Junction Protein 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Frozen

validated from a verified customer review.

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-85046 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tight Junction Protein 1

Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.

Alternate Names

DKFZp686M05161, MGC133289, Tight junction protein 1, tight junction protein 1 (zona occludens 1), tight junction protein ZO-1, TJP1, ZO1, ZO-1, zona occludens 1, Zona occludens protein 1, zonula occludens 1 protein, Zonula occludens protein 1

Gene Symbol

TJP1

Additional Tight Junction Protein 1 Products

Product Documents for Tight Junction Protein 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Tight Junction Protein 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Tight Junction Protein 1 Antibody - BSA Free

Customer Reviews for Tight Junction Protein 1 Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Tight Junction Protein 1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Rat intestine
    Species: Rat
    Verified Customer | Posted 05/21/2021
    Tight Junction expression (red color) in rat intestine. Blue color shows DAPI staining.
    Tight Junction Protein 1 Antibody - BSA Free NBP1-85046
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: RIPA whole cell lysates of mouse primary choroid plexus epithelial cells
    Species: Mouse
    Verified Customer | Posted 08/26/2015
    Murine choroid plexus primary cell lysate in RIPA
    Tight Junction Protein 1 Antibody - BSA Free NBP1-85046

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