Tight Junction Protein 2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-86850
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Scientific Data Images for Tight Junction Protein 2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850]
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850] - Green: CD31 Red: Tight Junction Protein 2 Blue: Nuclei. Lung frozen section was blocked and stained with TJP-2 at 1:100 dilution for a hour. A secondary Alexa 555-conjugated goat anti-rabbit antibody was used for labeling. Section was aso co-stained with CD31 antibody in conjunction with an Alexa 488-conjugated antibody. Nuclei was revealed by DAPU staining. Image submitted by a verified customer review.Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850]
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and cell junctions. Antibody staining is shown in green.Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850]
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining in primary mouse lung endothelial cells. Image submitted by a verified customer review.Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody - BSA Free [NBP1-86850]
Staining of human testis shows strong membranous and cytoplasmic positivity in cells in seminiferous ducts.Western Blot: Tight Junction Protein 2 Antibody [NBP1-86850]
Western Blot: Tight Junction Protein 2 Antibody [NBP1-86850] - Primary mouse lung endothelial cells treated with or without control IgG was blotted with the anti-TJP-2 antibody. Primary Antibody diluted 1:1000. Image submitted by a verified customer review.Immunoprecipitation: Tight Junction Protein 2 Antibody [NBP1-86850]
Immunoprecipitation: Tight Junction Protein 2 Antibody [NBP1-86850] - HEK293 cells were lysed and immunoprecipitated wtih anti-TJP2 ab (NBP1-86850) and Protein A/G PLUS-Agarose. The precipitates were separated by SDS-PAGE and blotted with anti-ZO1 antibody. IP image submitted by a verified customer review.Applications for Tight Junction Protein 2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Western Blot
Reviewed Applications
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Background: Tight Junction Protein 2
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Product Documents for Tight Junction Protein 2 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for Tight Junction Protein 2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Customer Reviews for Tight Junction Protein 2 Antibody - BSA Free (5)
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Application: ImmunoprecipitationSample Tested: 293 whole cell lysateSpecies: HumanVerified Customer | Posted 06/18/2021Western blotting result with immunoprecipitation experiments. HEK293 cells were lysed and immunoprecipitated wtih anti-TJP2 ab (NBP1-86850) and Protein A/G PLUS-Agarose. The precipitates underwent SDS-PAGE and blotted with anti-ZO1 antibody.Bio-Techne ResponseThis review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
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Application: Immunohistochemistry-ParaffinSample Tested: human skinSpecies: HumanVerified Customer | Posted 04/11/2019
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Application: ImmunocytochemistrySample Tested: Frozen Tissue and Mouse lung frozen sectionSpecies: MouseVerified Customer | Posted 05/17/2017Green: CD31 Red: Tight Junction Protein 2 Blue: NucleiLung frozen section was blocked and stained with TJP-2 @ 1:100 dilution for a hour. A secondary Alexa 555-conjugated goat anti-rabbit antibody was used for labeling. Section was aso co-stained with CD31 antibody in conjunction with an Alexa 488-conjugated antibody. Nuclei was revealed by DAPU staining.
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Application: Western BlotSample Tested: Primary mouse lung endothelial cellsSpecies: MouseVerified Customer | Posted 05/11/2017Primary mouse lung endothelial cells treated with or without control IgG was blotted with the anti-TJP-2 antibody.The antibody was diluted in PBST contained 5% milk at 1:1000. The secondary antibody was diluted at 1:5000.
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Primary mouse lung endothelial cellsSpecies: Primary mouse lung endothelial cells and MouseVerified Customer | Posted 10/13/2016The primary mouse lung endothelial cell was fixed, permeabilized and stained with 1:50 diluted anti-ZO-2 ab (NBP1-86850) for overnight. Cell was washed and was then incubated with Alexa flur546 dye for 1-hour in room temperature.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars