Tight Junction Protein 2 Antibody - BSA Free

4.6 out of 5

5 Customer Ratings

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• (5)

• Tight Junction Protein 2 Antibody

  Name: Akihiro Asai

  Application: Immunoprecipitation

  Sample Tested: 293 whole cell lysate

  Species: Human

  Verified Customer | Posted 06/18/2021

  Western blotting result with immunoprecipitation experiments. HEK293 cells were lysed and immunoprecipitated wtih anti-TJP2 ab (NBP1-86850) and Protein A/G PLUS-Agarose. The precipitates underwent SDS-PAGE and blotted with anti-ZO1 antibody.

  
  Bio-Techne Response

  This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

• Tight Junction Protein 2 Antibody

  Name: Anonymous

  Application: Immunohistochemistry-Paraffin

  Sample Tested: human skin

  Species: Human

  Verified Customer | Posted 04/11/2019

  

• Tight Junction Protein 2 Antibody

  Name: James Xiao

  Application: Immunocytochemistry

  Sample Tested: Frozen Tissue and Mouse lung frozen section

  Species: Mouse

  Verified Customer | Posted 05/17/2017

  Green: CD31 Red: Tight Junction Protein 2 Blue: Nuclei

  Lung frozen section was blocked and stained with TJP-2 @ 1:100 dilution for a hour. A secondary Alexa 555-conjugated goat anti-rabbit antibody was used for labeling. Section was aso co-stained with CD31 antibody in conjunction with an Alexa 488-conjugated antibody. Nuclei was revealed by DAPU staining.

  

• Tight Junction Protein 2 Antibody

  Name: James Xiao

  Application: Western Blot

  Sample Tested: Primary mouse lung endothelial cells

  Species: Mouse

  Verified Customer | Posted 05/11/2017

  Primary mouse lung endothelial cells treated with or without control IgG was blotted with the anti-TJP-2 antibody.

  The antibody was diluted in PBST contained 5% milk at 1:1000. The secondary antibody was diluted at 1:5000.

  

• Tight Junction Protein 2 Antibody

  Name: James Xiao

  Application: Immunocytochemistry/Immunofluorescence

  Sample Tested: Primary mouse lung endothelial cells

  Species: Primary mouse lung endothelial cells and Mouse

  Verified Customer | Posted 10/13/2016

  The primary mouse lung endothelial cell was fixed, permeabilized and stained with 1:50 diluted anti-ZO-2 ab (NBP1-86850) for overnight. Cell was washed and was then incubated with Alexa flur546 dye for 1-hour in room temperature.

  

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