TIMM9 Antibody (1D6) - Azide and BSA Free
Novus Biologicals | Catalog # H00026520-M01
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1D6
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
TIMM9 (AAH20213, 1 a.a. ~ 89 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAQIPESDQIKQFKEFLGTYNKLTETCFLDCVKDFTTREVKPEETTCSEHCLQKYLKMTQRISMRFQEYHIQQNEALAAKAGLLGQPR
Localization
Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.
Marker
Mitochondrial Biogenesis Marker
Specificity
TIMM9 - translocase of inner mitochondrial membrane 9 homolog (yeast)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for TIMM9 Antibody (1D6) - Azide and BSA Free
Western Blot: TIMM9 Antibody (1D6) [H00026520-M01]
Western Blot: TIMM9 Antibody (1D6) [H00026520-M01] - TIMM9 monoclonal antibody (M01), clone 1D6. Analysis of TIMM9 expression in NIH/3T3.Immunohistochemistry-Paraffin: TIMM9 Antibody (1D6) [H00026520-M01]
Immunohistochemistry-Paraffin: TIMM9 Antibody (1D6) [H00026520-M01] - Analysis of monoclonal antibody to TIMM9 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.Western Blot: TIMM9 Antibody (1D6) [H00026520-M01]
Western Blot: TIMM9 Antibody (1D6) [H00026520-M01] - TIMM9 monoclonal antibody (M01), clone 1D6. Analysis of TIMM9 expression in PC-12.Western Blot: TIMM9 Antibody (1D6) [H00026520-M01]
Western Blot: TIMM9 Antibody (1D6) [H00026520-M01] - TIMM9 monoclonal antibody (M01), clone 1D6 Analysis of TIMM9 expression in IMR-32.Western Blot: TIMM9 Antibody (1D6) [H00026520-M01]
Western Blot: TIMM9 Antibody (1D6) [H00026520-M01] - TIMM9 monoclonal antibody (M01), clone 1D6. Analysis of TIMM9 expression in Raw 264.7.Western Blot: TIMM9 Antibody (1D6) [H00026520-M01]
Western Blot: TIMM9 Antibody (1D6) [H00026520-M01] - Analysis of TIMM9 expression in transfected 293T cell line by TIMM9 monoclonal antibody (M01), clone 1D6.Lane 1: TIMM9 transfected lysate(10.4 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: TIMM9 Antibody (1D6) [H00026520-M01] - Detection limit for recombinant GST tagged TIMM9 is approximately 0.1ng/ml as a capture antibody.
Applications for TIMM9 Antibody (1D6) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Reviewed Applications
Read 1 review rated 4 using H00026520-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TIMM9
Alternate Names
TIM9Amitochondrial import inner membrane translocase subunit Tim9, TIM9TIMM9A, translocase of inner mitochondrial membrane 9 (yeast) homolog, translocase of inner mitochondrial membrane 9 homolog (yeast)
Entrez Gene IDs
26520 (Human)
Gene Symbol
TIMM9
OMIM
607384 (Human)
UniProt
Additional TIMM9 Products
Product Documents for TIMM9 Antibody (1D6) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TIMM9 Antibody (1D6) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for TIMM9 Antibody (1D6) - Azide and BSA Free
Customer Reviews for TIMM9 Antibody (1D6) - Azide and BSA Free (1)
4 out of 5
1 Customer Rating
Have you used TIMM9 Antibody (1D6) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Western BlotSample Tested: Human Glioma cells, whole cell lysatesSpecies: HumanVerified Customer | Posted 09/24/2023glioma cell treated with Control (lane 1), drugs (lane 2-7) for 3 days and subjected to WB. loaded protein: 10 ug primary Ab: 1/2000 dil in 2% BSA/TBST, for overnight at 4C. The bottom bands are TIMM9.
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...