TiterTACS In Situ Detection Kit - Colorimetric

Identification and quantitation of apoptosis in cultured cells in a 96 well assay
Catalog # Availability Size / Price Qty
4822-96-K
TiterTACS In Situ Detection Kit_4822-96-K
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TiterTACS In Situ Detection Kit - Colorimetric Summary

TUNEL based assay for high throughput quantitative detection of apoptosis.

Key Benefits

• Convenient, 96 well microplate format.
• Quantitative colorimetric detection.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

Why Use the TiterRACS In Situ Apoptosis Detection Kit?

During the process of apoptosis, DNA fragmentation occurs following the activation of endonucleases. The labeling of the 3’ ends of DNA fragments provides an easy measure of cells undergoing apoptosis. Modified nucleotides are incorporated at the 3’ ends by the activity of terminal deoxynucleotidyl transferase (TdT).

The TiterTACS Colorimetric Apoptosis Detection Kit is a TUNEL based assay utilizing specific in situ labeling technology that enables a 96 well microplate format for high throughput quantitative detection of apoptosis. TiterTACS can be used with suspension or monolayer cells. The kit is designed to use fixed samples, allowing you to work safely with samples that are infected with biohazardous agents. Fixed samples may be stored conveniently during time-course experiments. The TiterTACS Kit also provides TACS-Nuclease solution to generate positive controls from your own samples giving you a maximal value for the assay.

Detection using TACS-Sapphire, a non-toxic colorimetric substrate, allows both kinetic and endpoint readings. The reaction generates a blue product that can be measured at 630 nm, and will turn yellow after the reaction is stopped with acid allowing endpoint reading at 450 nm.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• Blue Strep-HRP Diluent
• TACS-Nuclease
• TACS-Nuclease Buffer
• TACS 2 TdT dNTP
• 50x Manganese Cation
• 10X TdT Labeling Buffer
• 10X TdT Stop Buffer
• TdT Enzyme
• TACS-Sapphire
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Detection of Apoptosis in Staurosporine-treated ML-1 Cells. Human ML-1 myeloblastic leukemia cells were treated with 1 µM staurosporine and then harvested, fixed, and labeled according to the protocol in the TiterTACS In Situ Detection Kit (Catalog # 4822-96-K) prior to colorimetric analysis.

Reagents Provided

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