TiterTACS In Situ Detection Kit - Colorimetric
TiterTACS In Situ Detection Kit - Colorimetric Summary
• Convenient, 96 well microplate format.
• Quantitative colorimetric detection.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
Why Use the TiterRACS In Situ Apoptosis Detection Kit?
During the process of apoptosis, DNA fragmentation occurs following the activation of endonucleases. The labeling of the 3’ ends of DNA fragments provides an easy measure of cells undergoing apoptosis. Modified nucleotides are incorporated at the 3’ ends by the activity of terminal deoxynucleotidyl transferase (TdT).
The TiterTACS Colorimetric Apoptosis Detection Kit is a TUNEL based assay utilizing specific in situ labeling technology that enables a 96 well microplate format for high throughput quantitative detection of apoptosis. TiterTACS can be used with suspension or monolayer cells. The kit is designed to use fixed samples, allowing you to work safely with samples that are infected with biohazardous agents. Fixed samples may be stored conveniently during time-course experiments. The TiterTACS Kit also provides TACS-Nuclease solution to generate positive controls from your own samples giving you a maximal value for the assay.
Detection using TACS-Sapphire, a non-toxic colorimetric substrate, allows both kinetic and endpoint readings. The reaction generates a blue product that can be measured at 630 nm, and will turn yellow after the reaction is stopped with acid allowing endpoint reading at 450 nm.
• Proteinase K
• Blue Strep-HRP Diluent
• TACS-Nuclease Buffer
• TACS 2 TdT dNTP
• 50x Manganese Cation
• 10X TdT Labeling Buffer
• 10X TdT Stop Buffer
• TdT Enzyme
For research use only. Not for diagnositic use.
Detection of Apoptosis in Staurosporine-treated ML-1 Cells. Human ML-1 myeloblastic leukemia cells were treated with 1 µM staurosporine and then harvested, fixed, and labeled according to the protocol in the TiterTACS In Situ Detection Kit (Catalog # 4822-96-K) prior to colorimetric analysis.
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