TiterTACS In Situ Detection Kit - Colorimetric

Identification and quantitation of apoptosis in cultured cells in a 96 well assay
Catalog # Availability Size / Price Qty
4822-96-K
TiterTACS In Situ Detection Kit_4822-96-K
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Product Details
Citations (28)
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TiterTACS In Situ Detection Kit - Colorimetric Summary

TUNEL based assay for high throughput quantitative detection of apoptosis.

Key Benefits

• Convenient, 96 well microplate format.
• Quantitative colorimetric detection.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

Why Use the TiterRACS In Situ Apoptosis Detection Kit?

During the process of apoptosis, DNA fragmentation occurs following the activation of endonucleases. The labeling of the 3’ ends of DNA fragments provides an easy measure of cells undergoing apoptosis. Modified nucleotides are incorporated at the 3’ ends by the activity of terminal deoxynucleotidyl transferase (TdT).

The TiterTACS Colorimetric Apoptosis Detection Kit is a TUNEL based assay utilizing specific in situ labeling technology that enables a 96 well microplate format for high throughput quantitative detection of apoptosis. TiterTACS can be used with suspension or monolayer cells. The kit is designed to use fixed samples, allowing you to work safely with samples that are infected with biohazardous agents. Fixed samples may be stored conveniently during time-course experiments. The TiterTACS Kit also provides TACS-Nuclease solution to generate positive controls from your own samples giving you a maximal value for the assay.

Detection using TACS-Sapphire, a non-toxic colorimetric substrate, allows both kinetic and endpoint readings. The reaction generates a blue product that can be measured at 630 nm, and will turn yellow after the reaction is stopped with acid allowing endpoint reading at 450 nm.

Kit Contents

• Proteinase K
• Streptavidin-HRP
• Blue Strep-HRP Diluent
• TACS-Nuclease
• TACS-Nuclease Buffer
• TACS 2 TdT dNTP
• 50x Manganese Cation
• 10X TdT Labeling Buffer
• 10X TdT Stop Buffer
• TdT Enzyme
• TACS-Sapphire
• Cytonin

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Data Example

Detection of Apoptosis in Staurosporine-treated ML-1 Cells. Human ML-1 myeloblastic leukemia cells were treated with 1 µM staurosporine and then harvested, fixed, and labeled according to the protocol in the TiterTACS In Situ Detection Kit (Catalog # 4822-96-K) prior to colorimetric analysis.

Citations for TiterTACS In Situ Detection Kit - Colorimetric

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. OGDHL is a modifier of AKT-dependent signaling and NF-kappaB function.
    Authors: Sen T, Sen N, Noordhuis M, Ravi R, Wu T, Ha P, Sidransky D, Hoque M
    PLoS ONE, 2102;7(11):e48770.  2102
  2. Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used
    Authors: L Haralambie, L Wien, N Gelbrich, J Lange, S Bakir, A Kramer, M Burchardt, A Ekkernkamp, D Gümbel, MB Stope
    Oncol Lett, 2020;19(1):283-290.  2020
  3. Piwi-Interacting RNA1037 Enhances Chemoresistance and Motility in Human Oral Squamous Cell Carcinoma Cells
    Authors: G Li, X Wang, C Li, S Hu, Z Niu, Q Sun, M Sun
    Onco Targets Ther, 2019;12(0):10615-10627.  2019
  4. Induced Tamoxifen Resistance is Mediated by Increased Methylation of E-Cadherin in Estrogen Receptor-Expressing Breast Cancer Cells
    Authors: Q Wang, M Gun, XY Hong
    Sci Rep, 2019;9(1):14140.  2019
  5. High hepatic expression of PDK4 improves survival upon multimodal treatment of colorectal liver metastases
    Authors: MJ Strowitzki, P Radhakrish, S Pavicevic, J Scheer, G Kimmer, AS Ritter, C Tuffs, C Volz, F Vondran, JM Harnoss, J Klose, T Schmidt, M Schneider
    Br. J. Cancer, 2019;0(0):.  2019
  6. Metformin Inhibit Lung Cancer Cell Growth and Invasion in Vitro as Well as Tumor Formation in Vivo Partially by Activating PP2A
    Authors: X Zhou, S Liu, X Lin, L Xu, X Mao, J Liu, Z Zhang, W Jiang, H Zhou
    Med. Sci. Monit., 2019;25(0):836-846.  2019
  7. Peptidoglycan-associated lipoprotein of Aggregatibacter actinomycetemcomitans induces apoptosis and production of proinflammatory cytokines via TLR2 in murine macrophages RAW 264.7 in vitro
    Authors: R Ihalin, K Eneslätt, S Asikainen
    J Oral Microbiol, 2018;10(1):1442079.  2018
  8. Combined Effect of Anticancer Agents and Cytochrome C Decorated Hybrid Nanoparticles for Liver Cancer Therapy
    Authors: W Al-Shakarc, A Alsuraifi, M Abed, M Abdullah, A Richardson, A Curtis, C Hoskins
    Pharmaceutics, 2018;10(2):.  2018
  9. Activated protein C suppresses osteoclast differentiation via endothelial protein C receptor, protease-activated receptor-1, sphingosine 1-phosphate receptor, and apolipoprotein E receptor 2
    Authors: K Yoshida, N Akita, T Okamoto, K Asanuma, A Uchida, A Sudo, M Shimaoka, K Suzuki, T Hayashi
    Thromb. Res., 2018;163(0):30-40.  2018
  10. Anti-ganglioside GD2 monoclonal antibody synergizes with cisplatin to induce endoplasmic reticulum-associated apoptosis in osteosarcoma cells
    Authors: W Zhu, X Mao, W Wang, Y Chen, D Li, H Li, P Dou
    Pharmazie, 2018;73(2):80-86.  2018
  11. A Novel Strategy to Prevent Advanced Atherosclerosis and Lower Blood Glucose in a Mouse Model of Metabolic Syndrome
    Authors: JE Kanter, F Kramer, S Barnhart, JM Duggan, M Shimizu-Al, V Kothari, A Chait, SD Bouman, JA Hamerman, BF Hansen, GS Olsen, KE Bornfeldt
    Diabetes, 2018;0(0):.  2018
  12. Cystic fibrosis epithelial cells are primed for apoptosis as a result of increased Fas (CD95)
    Authors: Q Chen, SPS Pandi, L Kerrigan, NG McElvaney, CM Greene, JS Elborn, CC Taggart, S Weldon
    J. Cyst. Fibros., 2018;0(0):.  2018
  13. Peptidoglycan-associated lipoprotein of Aggregatibacter actinomycetemcomitans induces apoptosis and production of proinflammatory cytokines via TLR2 in murine macrophages RAW 264.7 in vitro.
    Authors: Riikka Ihalin, Kjell Eneslätt, Sirkka Asikainen
    Journal of Oral Microbiology, 2018;0(0):2000-2297.  2018
  14. Beta Adrenergic Receptors Stimulation Attenuates Phosphorylation of NF-?B and I?B? in Hyperglycemic Endothelial Cells
    Authors: SZ Safi, H Shah, R Qvist, P Bindal, M Mansor, GOS Yan, ISB Ismail
    Cell. Physiol. Biochem., 2018;51(3):1429-1436.  2018
  15. The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival
    Authors: P Vange, T Bruland, B Doseth, R Fossmark, MML Sousa, V Beisvag, Ø Sørdal, G Qvigstad, HL Waldum, AK Sandvik, I Bakke
    PLoS ONE, 2017;12(9):e0184514.  2017
  16. Long noncoding RNA MALAT1 inhibits apoptosis induced by oxygen-glucose deprivation and reoxygenation in human brain microvascular endothelial cells
    Authors: JW Xin, YG Jiang
    Exp Ther Med, 2017;13(4):1225-1234.  2017
  17. Bmi1 essentially mediates podocalyxin-enhanced Cisplatin chemoresistance in oral tongue squamous cell carcinoma.
    Authors: Zhou Y, Zhang L, Pan H, Wang B, Yan F, Fang X, Munnee K, Tang Z
    PLoS ONE, 2015;10(4):e0123208.  2015
  18. Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease.
    Authors: Byun K, Bayarsaikhan E, Kim D, Kim CY, Mook-Jung I, Paek SH, Kim SU, Yamamoto T, Won MH, Song BJ, Park YM, Lee B
    PLoS ONE, 2012;7(5):e37917.  2012
  19. The P2Y6 Receptor Stimulates Bone Resorption by Osteoclasts.
    Authors: Orriss IR, Wang N, Burnstock G
    Endocrinology, 2011;152(10):3706-16.  2011
  20. Rubus coreanus Miq. extract promotes osteoblast differentiation and inhibits bone-resorbing mediators in MC3T3-E1 cells.
    Authors: Lee KH, Choi EM
    Am. J. Chin. Med., 2006;34(4):643-54.  2006
  21. Germline missense pathogenic variants in the BRCA1 BRCT domain, p.Gly1706Glu and p.Ala1708Glu, increase cellular sensitivity to PARP inhibitor olaparib by a dominant negative effect.
    Authors: Vaclova T, Woods N, Megias D, Gomez-Lopez S, Setien F, Garcia Bueno J, Macias J, Barroso A, Urioste M, Esteller M, Monteiro A, Benitez J, Osorio A
    Hum Mol Genet, 0;25(24):5287-5299.  0
  22. Inhibition of poly(ADP-Ribose) polymerase enhances the toxicity of 131I-metaiodobenzylguanidine/topotecan combination therapy to cells and xenografts that express the noradrenaline transporter.
    Authors: McCluskey A, Mairs R, Tesson M, Pimlott S, Babich J, Gaze M, Champion S, Boyd M
    J Nucl Med, 0;53(7):1146-54.  0
  23. ADAM12: a genetic modifier of preclinical peripheral arterial disease.
    Authors: Dokun A, Chen L, Okutsu M, Farber C, Hazarika S, Jones W, Craig D, Marchuk D, Lye R, Shah S, Annex B
    Am J Physiol Heart Circ Physiol, 0;309(5):H790-803.  0
  24. Detection and Quantification of Nuclear Morphology Changes in Apoptotic Cells by Fluorescence Microscopy and Subsequent Analysis of Visualized Fluorescent Signals.
    Authors: Mandelkow R, Gumbel D, Ahrend H, Kaul A, Zimmermann U, Burchardt M, Stope M
    Anticancer Res, 0;37(5):2239-2244.  0
  25. Diabetes promotes an inflammatory macrophage phenotype and atherosclerosis through acyl-CoA synthetase 1.
    Authors: Kanter J, Kramer F, Barnhart S, Averill M, Vivekanandan-Giri A, Vickery T, Li L, Becker L, Yuan W, Chait A, Braun K, Potter-Perigo S, Sanda S, Wight T, Pennathur S, Serhan C, Heinecke J, Coleman R, Bornfeldt K
    Proc Natl Acad Sci U S A, 0;109(12):E715-24.  0
  26. Hemin Causes Lung Microvascular Endothelial Barrier Dysfunction by Necroptotic Cell Death.
    Authors: Singla S, Sysol J, Dille B, Jones N, Chen J, Machado R
    Am J Respir Cell Mol Biol, 0;57(3):307-314.  0
  27. Visceral adipose tissue-derived serine proteinase inhibitor inhibits apoptosis of endothelial cells as a ligand for the cell-surface GRP78/voltage-dependent anion channel complex.
    Authors: Nakatsuka A, Wada J, Iseda I, Teshigawara S, Higashio K, Murakami K, Kanzaki M, Inoue K, Terami T, Katayama A, Hida K, Eguchi J, Ogawa D, Matsuki Y, Hiramatsu R, Yagita H, Kakuta S, Iwakura Y, Makino H
    Circ Res, 0;112(5):771-80.  0
  28. Desensitization of idiopathic pulmonary fibrosis fibroblasts to Alternaria alternata extract-mediated necrotic cell death.
    Authors: Im J, Kim K, Yhee J, O'Grady S, Nho R
    Physiol Rep, 0;4(21):.  0

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TiterTACS In Situ Detection Kit - Colorimetric
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