TLE1 Antibody (6Q5X1)

Novus Biologicals | Catalog # NBP3-16208

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6Q5X1 expressed in HEK293
View all TLE1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human TLE1 (Q04724). LQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPGTSNSLLVPDSLRGTDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TLE1 Antibody (6Q5X1)

Western Blot: TLE1 Antibody (6Q5X1) [NBP3-16208]

Western Blot: TLE1 Antibody (6Q5X1) [NBP3-16208]

Western Blot: TLE1 Antibody (6Q5X1) [NBP3-16208] - Western blot analysis of extracts of various cell lines, using TLE1 Rabbit mAb (NBP3-16208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: TLE1 Antibody (6Q5X1) [NBP3-16208]

Immunohistochemistry-Paraffin: TLE1 Antibody (6Q5X1) [NBP3-16208]

Immunohistochemistry-Paraffin: TLE1 Antibody (6Q5X1) [NBP3-16208] - Immunohistochemistry of paraffin-embedded mouse brain using TLE1 Rabbit mAb (NBP3-16208) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: TLE1 Antibody (6Q5X1) [NBP3-16208]

Western Blot: TLE1 Antibody (6Q5X1) [NBP3-16208]

Western Blot: TLE1 Antibody (6Q5X1) [NBP3-16208] - Western blot analysis of extracts of various cell lines, using TLE1 Rabbit mAb (NBP3-16208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for TLE1 Antibody (6Q5X1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TLE1

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG

Long Name

Transducin-like Enhancer of Split 1

Alternate Names

GRG1

Gene Symbol

TLE1

Additional TLE1 Products

Product Documents for TLE1 Antibody (6Q5X1)

Certificate of Analysis

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Product Specific Notices for TLE1 Antibody (6Q5X1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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