TLR8 Antibody (44C143) [PE]

Novus Biologicals | Catalog # NBP2-24817

Clone 44C143 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rabbit

Cited:

Human, Rabbit

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular)

Cited:

Flow Cytometry, Flow (Cell Surface), Flow - IC, Flow Cytometry Control, IF/IHC

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 44C143
View all TLR8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 21273544).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TLR8 Antibody (44C143) [PE]

TLR8-Antibody-44C143-PE-Flow-Cytometry-NBP2-24817-img0005.jpg
Flow Cytometry: TLR8 Antibody (44C143) [PE] [NBP2-24817] - Analysis of TLR8 in Ramos cells using 2 ug of NBP2-24817. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR8 antibody.
Flow Cytometry: TLR8 Antibody (44C143) [PE] [NBP2-24817] - Intracellular flow cytometric analysis of TLR8 in 10^6 human PBMCs using 0.5 ug of NBP2-24817, 0.25 ug of anti-human CD14, and 0.5 ug of isotype control. Novus's TLR intracellular staining flow assay kit was used to test this product.
Flow Cytometry: TLR8 Antibody (44C143) [PE] [NBP2-24817] - Intracellular analysis of TLR8 in BALB/c mouse splenocytes (lymphocyte gate) using 2 ug of this antibody. Green represents isotype control ; red represents anti-TLR8 antibody.

Applications for TLR8 Antibody (44C143) [PE]

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TLR8

Toll-like receptor 8 (TLR8) is a member of the TLR family of receptors that play a role in innate immune system activation and the recognition of pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs) (1,2). TLRs are type I membrane receptors that can be expressed on either the cell surface or internally, on endosomes (1,2). TLR8 is an endosomal receptor and is activated by pathogenic single stranded (ss) RNA (1-3). TLR8 is located on the X chromosome and is expressed mostly in monocytes/macrophages, neutrophils, and myeloid dendritic cells (1-3). Structurally, TLR8 consists of an extracellular domain, a cysteine-rich region, and transmembrane domain, and a Toll/Interleukin-1 receptor homology (TIR) domain (3,4). The extracellular domain contains a N-terminal leucine rich repeat (LRRNT) and a C-terminal LRR (LRRCT) which have 26 LRRs between them, each approximately 20-30 amino acids (aa), and a Z-loop between LRR14 and LRR15 (3). The primary isoform of the human TLR8 is synthesized as a protein 1041 aa in length with a theoretical molecular weight of ~120 kDa (4).

TLR8 is highly similar to TLR7 and both pathways are mediated by the adapter protein MyD88 to signal through IFN regulatory factor 7 (IRF7) and nuclear factor (NF)-kappaB (1-3,5). However, TLR7 recognizes guanosine and GU-rich ssRNA, while TLR8 recognizes uridine and AU-rich sequences (2,5). TLR7/TLR8 agonists, including derivatives of the immunostimulatory imiquimod, have been shown to be a promising cancer therapy capable of providing anticancer signals to antigen presenting cells (APCs), with many agonists being tested in both pre-clinical and clinical trials (6). Similarly, studies suggest that agonists for TLR8, in combination with other individual TLR agonists and antagonists, may also be useful for treating inflammatory allergic diseases, such as allergic rhinitis (7).

References

1. Sakaniwa, K., & Shimizu, T. (2020). Targeting the innate immune receptor TLR8 using small-molecule agents. Acta crystallographica. Section D, Structural biology, 76(Pt 7). https://doi.org/10.1107/S2059798320006518

2. Cervantes, J. L., Weinerman, B., Basole, C., & Salazar, J. C. (2012). TLR8: the forgotten relative revindicated. Cellular & molecular immunology. https://doi.org/10.1038/cmi.2012.38

3. Ohto, U., Tanji, H., & Shimizu, T. (2014). Structure and function of toll-like receptor 8. Microbes and infection. https://doi.org/10.1016/j.micinf.2014.01.007

4. Uniprot (Q9NR97)

5. Jannuzzi, G. P., de Almeida, J., Paulo, L., de Almeida, S. R., & Ferreira, K. S. (2020). Intracellular PRRs Activation in Targeting the Immune Response Against Fungal Infections. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2020.591970

6. Frega, G., Wu, Q., Le Naour, J., Vacchelli, E., Galluzzi, L., Kroemer, G., & Kepp, O. (2020). Trial Watch: experimental TLR7/TLR8 agonists for oncological indications. Oncoimmunology. https://doi.org/10.1080/2162402X.2020.1796002

7. Golshiri-Isfahani, A., Amizadeh, M., & Arababadi, M. K. (2018). The roles of toll like receptor 3, 7 and 8 in allergic rhinitis pathogenesis. Allergologia et immunopathologia. https://doi.org/10.1016/j.aller.2017.09.026

Long Name

Toll-like Receptor 8

Alternate Names

CD288

Gene Symbol

TLR8

Additional TLR8 Products

Product Documents for TLR8 Antibody (44C143) [PE]

Certificate of Analysis

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Product Specific Notices for TLR8 Antibody (44C143) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TLR8 Antibody (44C143) [PE]

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Protocols

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FAQs for TLR8 Antibody (44C143) [PE]

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  • Q: Do you have any FACS data for your Monoclonal Antibody to human TLR8 (Toll-like receptor 8)/CD288 PE Conjugate - showing that it doesn’t stain TLR8 negative cells? Is there a negative control other than the isotype control data?

    A:

    We don't test the antibody in a TLR8 negative cell model system (we don't have TLR8 knockout models). However, the specificity of the antibody clone (44C143) has been validated in the 2006 HDLA workshop. Here's a link with additional publications citing the antibody clone that are not yet listed on the data sheet: LINK.

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