TLR8 Antibody (44C143) - BSA Free

Novus Biologicals | Catalog # NBP2-24917

Clone 44C143 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Dot Blot, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Cytometric Bead Assay Standard, Flow - IC, Flow Cytometry Control, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 44C143

Format

BSA Free
View all TLR8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TLR8 Antibody (44C143) - BSA Free

Flow Cytometry: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]

Flow Cytometry: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]

TLR8-Antibody-44C143-Flow-Cytometry-NBP2-24917-img0018.jpg
Flow Cytometry: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]

Flow Cytometry: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]

TLR8-Antibody-44C143-Flow-Cytometry-NBP2-24917-img0021.jpg
Western Blot: TLR8 Antibody (44C143)BSA Free [NBP2-24917]

Western Blot: TLR8 Antibody (44C143)BSA Free [NBP2-24917]

TLR8-Antibody-44C143-Western-Blot-NBP2-24917-img0022.jpg
Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]

Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]

Immunohistochemistry-Paraffin: TLR8 Antibody (44C143) [NBP2-24917] - Formalin-fixed, paraffin-embedded human spleen stained with TLR8 antibody at 2 ug/ml.
Flow Cytometry: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]

Flow Cytometry: TLR8 Antibody (44C143) - BSA Free [NBP2-24917]

TLR8-Antibody-44C143-Flow-Cytometry-NBP2-24917-img0019.jpg
Western Blot: TLR8 Antibody (44C143)BSA Free [NBP2-24917]

Western Blot: TLR8 Antibody (44C143)BSA Free [NBP2-24917]

Western Blot: TLR8 Antibody (44C143) [NBP2-24917] - Analysis of TLR8 in human Ramos cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: TLR8 Antibody (44C143)BSA Free [NBP2-24917]

Simple Western: TLR8 Antibody (44C143)BSA Free [NBP2-24917]

Simple Western: TLR8 Antibody (44C143) [NBP2-24917] - Simple Western lane view shows a specific band for TLR8 in 0.5 mg/ml of Ramos (left) and Human Spleen (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody Image from the standard format of this antibody.

Applications for TLR8 Antibody (44C143) - BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature (PMID 27248820)

Flow (Cell Surface)

reported in multiple pieces of scientific literature

Flow (Intracellular)

reported in reported in multiple pieces of scientific literature (PMID 24836676)

Flow Cytometry

0.5-1 ug/10^6 cells

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

20 ug/ml

Western Blot

1-3 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. This antibody is CyTOF ready.
See Simple Western Antibody Database for Simple Western validation: Tested in Ramos and Human Spleen lysate 0.5 mg/mL, separated by Size, antibody dilution of 20 ug/mL

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR8

Toll-like receptor 8 (TLR8) is a member of the TLR family of receptors that play a role in innate immune system activation and the recognition of pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs) (1,2). TLRs are type I membrane receptors that can be expressed on either the cell surface or internally, on endosomes (1,2). TLR8 is an endosomal receptor and is activated by pathogenic single stranded (ss) RNA (1-3). TLR8 is located on the X chromosome and is expressed mostly in monocytes/macrophages, neutrophils, and myeloid dendritic cells (1-3). Structurally, TLR8 consists of an extracellular domain, a cysteine-rich region, and transmembrane domain, and a Toll/Interleukin-1 receptor homology (TIR) domain (3,4). The extracellular domain contains a N-terminal leucine rich repeat (LRRNT) and a C-terminal LRR (LRRCT) which have 26 LRRs between them, each approximately 20-30 amino acids (aa), and a Z-loop between LRR14 and LRR15 (3). The primary isoform of the human TLR8 is synthesized as a protein 1041 aa in length with a theoretical molecular weight of ~120 kDa (4).

TLR8 is highly similar to TLR7 and both pathways are mediated by the adapter protein MyD88 to signal through IFN regulatory factor 7 (IRF7) and nuclear factor (NF)-kappaB (1-3,5). However, TLR7 recognizes guanosine and GU-rich ssRNA, while TLR8 recognizes uridine and AU-rich sequences (2,5). TLR7/TLR8 agonists, including derivatives of the immunostimulatory imiquimod, have been shown to be a promising cancer therapy capable of providing anticancer signals to antigen presenting cells (APCs), with many agonists being tested in both pre-clinical and clinical trials (6). Similarly, studies suggest that agonists for TLR8, in combination with other individual TLR agonists and antagonists, may also be useful for treating inflammatory allergic diseases, such as allergic rhinitis (7).

References

1. Sakaniwa, K., & Shimizu, T. (2020). Targeting the innate immune receptor TLR8 using small-molecule agents. Acta crystallographica. Section D, Structural biology, 76(Pt 7). https://doi.org/10.1107/S2059798320006518

2. Cervantes, J. L., Weinerman, B., Basole, C., & Salazar, J. C. (2012). TLR8: the forgotten relative revindicated. Cellular & molecular immunology. https://doi.org/10.1038/cmi.2012.38

3. Ohto, U., Tanji, H., & Shimizu, T. (2014). Structure and function of toll-like receptor 8. Microbes and infection. https://doi.org/10.1016/j.micinf.2014.01.007

4. Uniprot (Q9NR97)

5. Jannuzzi, G. P., de Almeida, J., Paulo, L., de Almeida, S. R., & Ferreira, K. S. (2020). Intracellular PRRs Activation in Targeting the Immune Response Against Fungal Infections. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2020.591970

6. Frega, G., Wu, Q., Le Naour, J., Vacchelli, E., Galluzzi, L., Kroemer, G., & Kepp, O. (2020). Trial Watch: experimental TLR7/TLR8 agonists for oncological indications. Oncoimmunology. https://doi.org/10.1080/2162402X.2020.1796002

7. Golshiri-Isfahani, A., Amizadeh, M., & Arababadi, M. K. (2018). The roles of toll like receptor 3, 7 and 8 in allergic rhinitis pathogenesis. Allergologia et immunopathologia. https://doi.org/10.1016/j.aller.2017.09.026

Long Name

Toll-like Receptor 8

Alternate Names

CD288

Gene Symbol

TLR8

Additional TLR8 Products

Product Documents for TLR8 Antibody (44C143) - BSA Free

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Product Specific Notices for TLR8 Antibody (44C143) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TLR8 Antibody (44C143) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TLR8 Antibody (44C143) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

    A: These antibodies are all purified from tissue culture supernatant.

  • Q: Do you have any reference paperd to show this antibody (clone 44C143) reacts with mouse tissues?

    A:

    Yes we do indeed have several papers cited with use in Mouse. I am not certain about your application so I am going to note the links to all of the papers that used the antibody in mouse.
    http://www.ncbi.nlm.nih.gov/pubmed/16446426
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365979/
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518890/
    I also have performed a blast comparison between the human immunogen sequence and the mouse and found it has 70 % homology. we would recommend the homology to be higher than 85% for predicting whether an antibody will work however there must be enough homology in the actual epitope.

  • Q: Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

    A: These antibodies are all purified from tissue culture supernatant.

  • Q: Do you have any reference paperd to show this antibody (clone 44C143) reacts with mouse tissues?

    A:

    Yes we do indeed have several papers cited with use in Mouse. I am not certain about your application so I am going to note the links to all of the papers that used the antibody in mouse.
    http://www.ncbi.nlm.nih.gov/pubmed/16446426
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365979/
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518890/
    I also have performed a blast comparison between the human immunogen sequence and the mouse and found it has 70 % homology. we would recommend the homology to be higher than 85% for predicting whether an antibody will work however there must be enough homology in the actual epitope.

Showing  1 - 2 of 2 FAQs Showing All
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