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Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunoprecipitation, Dot Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # MTn-12
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Product Specifications
Immunogen
Partially purified mouse tenascin.
Reactivity Notes
. No cross reactivity with tenascin of other species has been observed.
Localization
In immunohistological testing of frozen tissue sections of mouse intestine, the antibody stains the core of the villi but not the epithelial cells.
Specificity
This antibody localizes mouse tenascin in the supernatant of cultured mouse fibroblasts and tissue extracts. No cross-reactivity with tenascin of other species has been observed. In immunohistological testing of frozen tissue sections of mouse intestine, the antibody labels the core of the villi, but not the epithelial cells. In supernatants of cultured mouse fibroblasts, the antibody precipitates two polypeptides of approximate molecular weights 210 and 260 kDa. It also recognizes these tenascin chains and the 230 kDa endoglycosidase F-digested fragment in immunoblotting of tissue extracts and fibroblasts lysates under reducing conditions.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for TNN Antibody (MTn-12)
Immunohistochemistry-Frozen: TNN Antibody (MTn-12) [NBP3-08210]
Immunohistochemistry-Frozen: TNN Antibody (MTn-12) [NBP3-08210] - Frozen sections, acetone fixed of mouse intestine were stained with 1:400 monoclonal TNN Antibody (Clone: MTn-12) followed by 1:20 monoclonal Anti-Rat Kappa & Lambda Light Chains-FITC antibody produced in mouse and by 1:20 Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat.Applications for TNN Antibody (MTn-12)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:200
Immunohistochemistry-Frozen
1:200
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
IHC-Fr: In immunohistological testing of frozen tissue sections of mouse intestine, using immunofluorescent or immunoperoxidase staining, the antibody stains the core of the villi but not the epithelial cells. Dilution: 1:200.
ELISA: In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I.
ELISA: In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.09% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TNN
Alternate Names
tenascin N, tenascin-N, tenascin-W, TN-N, TNW, TN-W
Gene Symbol
TNN
Additional TNN Products
Product Documents for TNN Antibody (MTn-12)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TNN Antibody (MTn-12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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