TOE1 Antibody (1D8) - Azide and BSA Free
Novus Biologicals | Catalog # H00114034-M01
![Western Blot: TOE1 Antibody (1D8) [H00114034-M01]](https://resources.rndsystems.com/images/products/TOE1-Antibody-1D8-Western-Blot-H00114034-M01-img0008.jpg "Western Blot: TOE1 Antibody (1D8) [H00114034-M01]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1D8
Format
Azide and BSA Free
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Product Specifications
Immunogen
TOE1 (AAH09364, 1 a.a. ~ 510 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAADSDDGAVSAPAASDGGVSKSTTSGEELVVQVPVVDVQSNNFKEMWPSLLLAIKTANFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSILSLGLACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQFLIQHGFNFNQQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSSMRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAEDKRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNLPHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWGSS
Specificity
TOE1 - target of EGR1, member 1 (nuclear)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TOE1 Antibody (1D8) - Azide and BSA Free
Western Blot: TOE1 Antibody (1D8) [H00114034-M01]
Western Blot: TOE1 Antibody (1D8) [H00114034-M01] - TOE1 monoclonal antibody (M01), clone 1D8 Analysis of TOE1 expression in Jurkat.![Immunohistochemistry-Paraffin: TOE1 Antibody (1D8) [H00114034-M01]](https://resources.rndsystems.com/images/products/TOE1-Antibody-1D8-Immunohistochemistry-Paraffin-H00114034-M01-img0006.jpg "Immunohistochemistry-Paraffin: TOE1 Antibody (1D8) [H00114034-M01]")
Immunohistochemistry-Paraffin: TOE1 Antibody (1D8) [H00114034-M01]
Immunohistochemistry-Paraffin: TOE1 Antibody (1D8) [H00114034-M01] - Analysis of monoclonal antibody to TOE1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.3 ug/ml.![Western Blot: TOE1 Antibody (1D8) [H00114034-M01]](https://resources.rndsystems.com/images/products/TOE1-Antibody-1D8-Western-Blot-H00114034-M01-img0007.jpg "Western Blot: TOE1 Antibody (1D8) [H00114034-M01]")
Western Blot: TOE1 Antibody (1D8) [H00114034-M01]
Western Blot: TOE1 Antibody (1D8) [H00114034-M01] - Analysis of TOE1 over-expressed 293 cell line, cotransfected with TOE1 Validated Chimera RNAi ( Cat # H00114034-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOE1 monoclonal antibody (M01), clone 1D8 (Cat # H00114034-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: TOE1 Antibody (1D8) [H00114034-M01]](https://resources.rndsystems.com/images/products/TOE1-Antibody-1D8-Western-Blot-H00114034-M01-img0009.jpg "Western Blot: TOE1 Antibody (1D8) [H00114034-M01]")
Western Blot: TOE1 Antibody (1D8) [H00114034-M01]
Western Blot: TOE1 Antibody (1D8) [H00114034-M01] - Analysis of TOE1 expression in transfected 293T cell line by TOE1 monoclonal antibody (M01), clone 1D8.Lane 1: TOE1 transfected lysate(56.5 KDa).Lane 2: Non-transfected lysate.Applications for TOE1 Antibody (1D8) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA, RNAi validation, and immunohistochemistry (paraffin).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TOE1
Alternate Names
FLJ13949, target of EGR1 protein 1, target of EGR1, member 1 (nuclear)
Entrez Gene IDs
114034 (Human)
Gene Symbol
TOE1
UniProt
Additional TOE1 Products
Product Documents for TOE1 Antibody (1D8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TOE1 Antibody (1D8) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for TOE1 Antibody (1D8) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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