TOMM20 Antibody (4F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00009804-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F3

Format

Azide and BSA Free
View all TOMM20 Primary Antibodies »

Product Specifications

Immunogen

TOMM20 (AAH66335, 1 a.a. ~ 145 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE

Localization

Mitochondrion outer membrane; Multi-pass membrane protein

Specificity

TOMM20 - translocase of outer mitochondrial membrane 20 homolog (yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TOMM20 Antibody (4F3) - Azide and BSA Free

Western Blot: TOMM20 Antibody (4F3) [H00009804-M01]

Western Blot: TOMM20 Antibody (4F3) [H00009804-M01]

Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of TOMM20 expression in transfected 293T cell line by TOMM20 monoclonal antibody (M01), clone 4F3.Lane 1: TOMM20 transfected lysate(16.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (4F3) [H00009804-M01]

Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (4F3) [H00009804-M01]

Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of monoclonal antibody to TOMM20 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry: TOMM20 Antibody (4F3) [H00009804-M01]

Immunohistochemistry: TOMM20 Antibody (4F3) [H00009804-M01]

TOMM20-Antibody-4F3-Immunohistochemistry-H00009804-M01-img0018.jpg
Western Blot: TOMM20 Antibody (4F3) [H00009804-M01]

Western Blot: TOMM20 Antibody (4F3) [H00009804-M01]

Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - TOMM20 monoclonal antibody (M01), clone 4F3 Analysis of TOMM20 expression in HeLa.
Western Blot: TOMM20 Antibody (4F3) [H00009804-M01]

Western Blot: TOMM20 Antibody (4F3) [H00009804-M01]

Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - TOMM20 monoclonal antibody (M01), clone 4F3. Analysis of TOMM20 expression in PC-12.
Western Blot: TOMM20 Antibody (4F3) [H00009804-M01]

Western Blot: TOMM20 Antibody (4F3) [H00009804-M01]

Western Blot: TOMM20 Antibody (4F3) [H00009804-M01] - TOMM20 monoclonal antibody (M01), clone 4F3. Analysis of TOMM20 expression in NIH/3T3.
Immunohistochemistry-Paraffin: TOMM20 Antibody (4F3) [H00009804-M01]

Immunohistochemistry-Paraffin: TOMM20 Antibody (4F3) [H00009804-M01]

Immunohistochemistry-Paraffin: TOMM20 Antibody (4F3) [H00009804-M01] - Analysis of monoclonal antibody to TOMM20 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
ELISA: TOMM20 Antibody (4F3) [H00009804-M01]

ELISA: TOMM20 Antibody (4F3) [H00009804-M01]

ELISA: TOMM20 Antibody (4F3) [H00009804-M01] - Detection limit for recombinant GST tagged TOMM20 is approximately 0.1ng/ml as a capture antibody.
TOMM20 Antibody (4F3)

Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (4F3) [H00009804-M01] -

Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (4F3) [H00009804-M01] - Upregulation of GAPDH & Tom20 in C6 gliomas. a A section of rat brain stained with hematoxilin & eosin to show a glioma produced by implantation of C6 cells in the right caudate nucleus. In this case, cells also grew in the region of the syringe needle track through the cortex. (b–d). A small glioma labeled by bisbenzamide (b) & with antibodies against Tom20 (c) & GAPDH (d). The brightness & contrast have been increased. e Illustration of a strip perpendicular to the rim of a glioma (labeled with bisbenzamide) & along which intensity profiles were measured. f, g Tom20 & GAPDH fluorescence along such a strip (barely visible without enhancement). The graphs show the raw intensity values given by ImageJ. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26032618), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TOMM20 Antibody (4F3) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Use in FLOW cytometry reported in scientific literature ( PMID 26779811).

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TOMM20

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore

Long Name

TOMM20

Alternate Names

MAS20, mitochondrial 20 kDa outer membrane protein, mitochondrial import receptor subunit TOM20 homolo, MOM19, outer mitochondrial membrane receptor Tom20, TOM20, translocase of outer mitochondrial membrane 20 hom

Entrez Gene IDs

9804 (Human)

Gene Symbol

Q15388

OMIM

601848 (Human)

UniProt

AAH66335 (Human)

Additional TOMM20 Products

Product Documents for TOMM20 Antibody (4F3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TOMM20 Antibody (4F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TOMM20 Antibody (4F3) - Azide and BSA Free

Customer Reviews for TOMM20 Antibody (4F3) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review TOMM20 Antibody (4F3) - Azide and BSA Free and earn rewards!

Have you used TOMM20 Antibody (4F3) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies